摘要
目的 利用人胃癌细胞株SGC7901细胞,探讨乙酰肝素酶反义寡核苷酸对人胃癌细胞株SGC7901中表达碱性成纤维细胞生长因子(bFGF)的影响。方法 采用脂质体介导乙酰肝素酶反义寡核苷酸转染人胃癌细胞株SGC7901细胞,采用免疫细胞化学法观察细胞内bFGF的变化。结果 脂质体介导转染乙酰肝素酶反义寡核苷酸后,SGC7901胃癌细胞内乙酰肝素酶mRNA表达下降,bF GF阳性表达率由转染前的 72 23%降至 40 25%。转染后细胞bFGF表达明显减少。结论 转染乙酰肝素酶反义寡核苷酸可以有效抑制胃癌细胞bFGF的表达。
Objective To evaluate the effect of heparanase antisense oligodeoxynucleotide (AS-OND) on the expression of basic fibroblast growth factor (bFGF) in human gastric carcinoma cell line SGC 7901.Metheds The AS-ODN complementary to the start codon region of heparanase mRNA and its control,scrambled nonsense oligodeoxynucleotide (NS-ODN) were designed and synthesized and phosphorothioated.The ODNs were embedded in cationic liposome lipofectin and transfected into SGC 7901 cells.The total RNAs were extracted from the cells 48 hours after transfection and the semi-quantitative RT-PCR were performed to evaluate the heparanase gene.The change of bFGF was observed by using immunocytochemistry.Results The heparanase gene expression and the expression of bFGF of SGC 7901 cells treated with AS-ODN in different final concentrations were significantly decreased comparing with that of the controls (P<001).The expression rates were 72.23%,57.15%,51.11%,42.36% and 40.25% at cells before and after treated with AS-ODN in the final concentrations of 0.1 μmol/L,0.2 μmol/L,0.4 μmol/L and 0.8 μmol/L,respectively.Conclusion Heparanase AS-ODN complementary to the start codon region of heparanase mRNA has a significant effect on the expression of bFGF in human gastric carcinoma cell line SGC 7901 in a dose-depended manner.
出处
《临床消化病杂志》
2005年第1期5-7,共3页
Chinese Journal of Clinical Gastroenterology
关键词
乙酰肝素酶
碱性成纤维细胞生长因子
反义寡核苷酸
胃癌
Heparanase
Basic fibroblast growth factor
Antisense oligodeoxynucleotide
Gastric carcinoma
