摘要
目的 采用RP HPLC测定盐酸青藤碱灌胃和皮肤给药后不同时间大鼠体内血清浓度 ,计算出灌胃和皮肤给药途径主要药动学参数。方法 采用RP HPLC以乙腈 0 .0 1mol·L-1磷酸二氢钠溶液 四甲基二乙胺 (46∶5 4∶0 . 2 2 )为流动相 ,磷酸调至pH6 9,C18ODS柱 (4. 6mm× 2 5 .0mm ,5 μm) ,紫外检测波长为 2 6. 3nm。 结果 盐酸青藤碱在 0 . 2 5～ 10 . 0 μg·mL-1范围内呈良好线性关系 (r=0 . 9995 ) ,方法的平均回收率达 98 .5 % ,RSD =1 3%。计算出灌胃给药途径主要药动学参数 :Ke0 31h-1,AUC 18 .0 7μg·h·mL-1,t1/ 2 2 . 2 4h ,cmax6 . 2 6 μg·mL-1,tmax0 . 75h ;皮肤给药途径主要药动学参数 :Ke0 . 92h-1,AUC 3 2 .4 μg·h·mL-1,t1/ 2 0 . 75h ,cmax1. 17μg·mL-1,tmax1h。结论 本法分离效果好 ,分析速度快 ,方法稳定 ,结果准确。
OBJECTIVE: A RP-HPLC assay was developed to determine the concentration of sinomenine HCl in serum and its pharmacokinetics was studied in rats after ig and skin administration. METHODS: C18H37 column was eluted with the mobile phase of acetonitrile - 0.01 mol·L-1 sodium phosphate monobasic-N,N,N′,N′-tetramethlenediamine(46:54:0.22.pH 6.9) and the ultraviolet wavelength was at 263 run. RESULTS: The assay was linear within the range of 0.25-10.0 μg·mL-1, (r = 0.999 5). The mean recovery was 98.5% with RSD 1.3%. The pharmacokinetic parameters of sinomenine HCl in rats were obtained after two administration. The pharmacokinetic parameters after an intragastric administration included: Ke was 0.31, AUC was 18.07 μg·h·mL-1, t1/2 was 2.24 h, cmax was 6.26 μg·mL-1, tmax was 0.75 h. The pharmacokinetic parameters after a subcutanous administration included: Ke was 0.92, AUC was 3.24 μg·h·mL-1, t1/2 was 0.75 h, Cmax was 1.17 μg·mL -1, tmax was 1 h. CONCLUSION: This method was easy, rapid, accurate and sensitive.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第1期54-55,共2页
Chinese Pharmaceutical Journal
关键词
盐酸青藤碱
高效液相色谱法
药动学
Acetonitrile
Body fluids
High performance liquid chromatography
Living systems studies
Pharmacokinetics
Skin
