摘要
用富集-分离-筛选的方法从花园土壤中分离纯化得到两株具有较高絮凝活性的絮凝剂产生菌。通过研究2B菌株在不同培养时间的生长情况、培养液中pH值变化情况及絮凝活性等,从而得出絮凝活性与菌生长量成正相关,且该菌产生的絮凝剂对高岭土悬浊液具有较高的絮凝活性和稳定性。环境因素对絮凝剂产生的影响研究表明,2B菌株产絮凝剂的最佳培养基为查氏培养基;菌产絮凝剂的最佳培养条件:初始pH值为7～9,培养温度30℃,培养时间为72h,通气量为150r/min。研究表明,2B菌株所产絮凝剂絮凝高岭土悬浊液的最适宜pH在大于7的偏碱性条件下,Ca2+、Mg2+和Al3+等金属离子对其絮凝具有促进作用,絮凝率可高达94.6%。
Two flocculants-producing strains of high flocculating activity were screened from the soil by the method of enrichment-isolation-screening.The growth of the strains, flocculating activity and pH at different time during the culture of the strains was studied and four different kinds of media were selected to culture the microorganisms.It is found that zapek medium is one to achieve the highest production rate at a low cost under the optimum conditions: pH values within 7 to 9, culture temperature 30℃ and cultivation for 72 h.It is indicated that the suitable pH value for the microbial flocculants to flocculate Kaolin clay suspension is higher than 7 and the flocculation ratio is up to 94.6% with the synergy of Ca^(2+)、 Mg^(2+) and Al^(3+).
出处
《重庆建筑大学学报》
CSCD
2004年第6期63-66,共4页
Journal of Chongqing Jianzhu University
基金
重庆大学骨干教师资助计划(71641104)
关键词
微生物絮凝剂
絮凝剂产生菌
絮凝活性
时间曲线
microbial flocculants
flocculants-producing microorganism
flocculation activity
time curve
