摘要
目的 探讨丙戊酸钠 (VAP)对戊四氮 (PTZ)致疒间 大鼠海马Bax和Bcl 2表达的影响。方法 将2 4只成年Wistar大鼠随机分为正常对照 (NC)组、PTZ组和VAP组 ,PTZ组和VAP组大鼠腹腔注射阈下剂量的PTZ 35mg/ (kg·d) ,直至达到点燃标准。点燃后 ,VAP组大鼠经腹腔注入VAP 15mg/ (kg·d) ,PTZ组大鼠经腹腔注入生理盐水 ,30min后 ,再腹腔注射PTZ诱发癫疒间 发作。应用免疫组化法检测大鼠海马神经元Bax和Bcl 2蛋白的表达。结果 大鼠海马神经元Bax阳性细胞数和光密度 ,PTZ组均明显高于VAP组和NC组 (均P <0 0 1) ,VAP组明显高于NC组 (P <0 0 5 ) ;大鼠海马神经元Bcl 2阳性细胞数和光密度 ,PTZ组明显高于NC组(P <0 0 5 ) ,VAP组均明显高于PTZ组和NC组 (均P <0 0 1)。结论 VAP可以增强癫疒间大鼠海马神经元Bcl 2的表达和降低Bax的表达 ,有对抗癫疒间 发作导致细胞凋亡的作用。
Objective To explore the effect of vaproate sodium (VP) on Bcl-2 and Bax expression of hippocampus in seizures rats induced by pentylenetetrazole(PTZ).Methods Twenty-four adult Wistar rats were randomly divided into normal control (NC) group, PTZ group and VAP group. A dose of PTZ [35 mg/(kg·day)] was intraperitoneally injected into the rats of PTZ group and VAP group until the kindling criterion was reached. After kindling, the rats of VAP group were administered intraperitoneally with VAP[15 mg/(kg·day)];the rats of PTZ group were administered intraperitoneally with normal saline. After 30 minutes, seizures were induced by administering PTZ intraperitoneally. The influence of VAP on Bcl-2 and Bax immmunoreactivity on hippocampus neurons was studied by immunohistochemistry method.Results Bax positive cells in hippocampus in PTZ group were more than in VAP group and NC group(all P< 0.01). Furthermore, Bax positive cells in VAP group were more than in NC group( P< 0.05). Bcl-2 positive cells in hippocampus in PTZ group were more than in NC group( P< 0.05), and these cells in VAP group were more than in PTZ group and NC group(all P< 0.01).Conclusion Vaproate sodium can resist the apoptosis of hippocampus neurons in seizures rats by increased expression of Bcl-2 and decreased expression of Bax.
出处
《临床神经病学杂志》
CAS
2004年第5期351-353,共3页
Journal of Clinical Neurology
