摘要
以铺散诸葛菜的叶柄及叶片为外植体 ,研究了不同的再生方式。在 6 BA和NAA配合使用的MS培养基上 ,叶柄和叶片外植体有高的成芽率 ,具有强的器官再生能力。在 2 4 D与 6 BA、NAA结合的培养基上 ,叶柄和叶片有较高的愈伤组织诱导率 ,在含 6 BA的培养基上也易于分化。叶柄、叶片再生的芽和经愈伤组织分化而得来的芽快速繁殖后 ,在生根培养基上形成完整植株。研究表明铺散诸葛菜具有极强的器官分化能力。
The different ways of regeneration of leaves and petioles in vitro were studied in Orychophragmus diffusus Z.M.Tan et J.M.Xu. On the MS medium with 6-BA and NAA, the leaves and petioles gave the high regeneration rate. When they were cultured on the MS medium with 24-D,6-BA and NAA, they gave the high induction rate of the calli. The calli were differentiated easily on the medium with 6-BA. The embryonic plants were reproduced rapidly in the same medium. Complete plants were induced on the rooting medium. This study showed O.diffusus has apparent regeneration capacity.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第5期679-681,共3页
Acta Horticulturae Sinica
