摘要
目的 采用酶联免疫吸附测定 (ELISA)技术检测疟疾媒介按蚊环子孢子蛋白 (CSP) ,评价ELISA用于云南疟疾媒介按蚊传疟效能调查的可行性。 方法 现场捕获疟疾媒介按蚊经产蚊 ,每只蚊镜检 3叶唾腺子孢子感染率 ,ELISA检测另 3叶唾腺CSP ;用含间日疟原虫配子体的患者血人工喂饲微小按蚊 ,11d后同法镜检唾腺子孢子感染率及ELISA检测唾腺CSP。在种植场捕获 8种按蚊 (微小按蚊、中华按蚊、多斑按蚊、迷糊按蚊、可赫按蚊、带足按蚊、菲律宾按蚊和须喙按蚊 )各龄成蚊 ,ELISA检测唾腺CSP。 结果 ①现场捕获经产蚊 10 10只 ,镜检唾腺子孢子阳性 7只 ,阳性率为 0 69% ;ELISA检测唾腺CSP阳性 8只 ,阳性率为 0 79% ;其中 6只蚊两项检测均为阳性 ,阳性率为 0 5 9%。两法比较 ,差异无显著性意义 (P >0 0 5 )。②人工感染 3 6只微小按蚊 ,镜检唾腺子孢子阳性2 7只 ,阳性率为 75 0 % ;ELISA检测唾腺CSP阳性 2 9只 ,阳性率为 80 6% ;其中有 2 6只蚊两项检测 (Pv2 10CSP)均阳性 ,阳性率为 72 2 %。两法比较 ,差异无显著性意义 (P >0 0 5 )。③种植场捕获 8种按蚊各龄成蚊 4675只 ,ELISA检测唾腺CSP阳性 11只 ,阳性率为 0 2 4% ;其中Pv2 10阳性 9只 ,Pf2A10阳性 2只 ;微小按蚊。
Objective To detect circumsporozoite protein (CSP) in anopheline vectors from south Yunnan and to evaluate ELISA in the detection. \ Methods\ Salivary glands of the anopheline mosquitoes were taken for finding sporozoites by microscopy and part of the glands was used for detecting CSP by ELISA. An. minimus was experimentally infected by blood from vivax malaria patient (with Plasmodium vivax) and examined for sporozoites and CSP. Eight species of anopheline mosquitoes were caught in the field and examined. Monoclonal antibodies to P.falciparum (Pf2A10) and P.vivax (Pv210, Pv247) were used in ELISA for detecting CSP. \ Results\ Sporozoites were found in the salivary glands of 27 out of 36 An. minimus experimentally infected (75\^0%), 29 were ELISA CSP positives (80\^6%), and 26 of the 27 mosquitoes showed Pv210 CSP positive. Among \{1 010\} parous anopheline mosquitoes from the field, 7 were found sporozoite positive (0\^69%), 8 were ELISA CSP positive (0\^79%), and 6 of the 7 mosquitoes showed CSP positive. Of \{4 675\} wild mosquitoes in 8 anopheline species with different ages, 11 were found CSP positive (0\^24%) including An.minimus, An.sinensis and An.maculatus with a positive rate of 0\^20%, 0\^24% and 0\^39% respectively.Among the 11 mosquitoes, 9 were Pv210 positive and 2 were Pf2A10 positive. Conclusion CSP detection by ELISA is a useful method to monitor the malaria transmission capacity of anopheline vectors.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2004年第4期227-230,共4页
Chinese Journal of Parasitology and Parasitic Diseases
