摘要
目的研究低热高压法制作的聚丙交酯-乙交酯(PLGA)支架在体外降解后生物力学和固有粘滞系数等的变化.方法将空隙率为90.0%和92.5%的PLGA在37℃生理盐水中降解观察8周,每周进行体积、质量丢失、粘滞系数衰减和抗压强度测试,同时观察降解液酸度变化.结果两组支架的质量丢失差异显著,各组粘滞系数在第1周后逐渐衰减,至第6周时衰减一半.支架抗压强度自第4周起减至原来的1/2,外形在4～8周观察期间发生显著坍塌.降解液中的pH值在整个过程中保持在6.0～6.5之间,在前4周90.0%组比92.5%组pH值低,其后没有差别.结论低热高压法制作的PLGA支架生物力学性能是稳定的,半衰期为6周,适于在组织工程实验中应用.
Objective To study the changes in biomechanics a nd such indices as intrinsic viscosity poly (lactide-co-glycolide) (PLGA) scaffold s produced by mild heating under high pressure after in vitro degradation. Metho d PLGA scaffolds with the porosity of 90.0% and 92.5% respectively were immerge d in 37 ℃saline for 8 weeks, and the changes in their mass, intrinsic viscosit y and loss of compressive strength were assessed on a weekly basis, and the acid ity of the degradation solution was also measured regularly. Results Significa nt differences was noted in the mass reduction between the scaffolds, and the i ntrinsic viscosity began to decrease in both groups in the first week to half of the original value till the sixth week. A 50% reduction in the compressive str ength of the scaffolds occurred at the fourth week, and till the eighth week, ob vious structural collapse was observed. Along with the changes, the acidity of the degradation solution increased from 6.0 to 6.5, and the solution of 90.0% p orosity group had lower pH value during the first 4 weeks than 92.5% porosity gr oup, but such difference was no longer seen afterwards. Conclusion PLGA s caffolds made by mild heating under high pressure have stable biomecha nical performance with the half-life of approximately 6 weeks, which can be appl icable for tissue engineering.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第5期416-420,共5页
Journal of First Military Medical University
基金
国家自然科学基金(30170953)
广东省重点科技攻关项目(2KM0505S)~~
