摘要
本实验研究了不同温度(15℃～35°C)对青蒿毛状根生长和青蒿素生物合成的影响,发现25°C有利于毛状根生长,30°C促进了青蒿素生物合成。通过温度改变的二步培养技术(培养前20d温度控制在25°C,后10d温度提高到30°C),青蒿素的产量得到明显提高,高于在恒温培养时(25°C或30°C)的结果。
Temperature effect on growth and artemisinin biosynthesis in the cultured Artemisia annua L. hairy roots was investigated at a range of 15°C ~ 35°C. Maximum hairy root growth was found at 25°C, however, the highest artemisinin content in the root cultures was observed at 30°C. Based on these results, a step-wise temperature shift strategy was developed in which 25°C was used for cultivation for the first 20 days and 30°C for the remainder of the cultivation period. The artemisinin production obtained by the strategy was higher than those in single temperature-control experiments.
出处
《西北植物学报》
CAS
CSCD
2004年第10期1828-1831,共4页
Acta Botanica Boreali-Occidentalia Sinica
关键词
青蒿
青蒿素
毛状根
温度
Artemisia annua L.
artemisinin
hairy root cultures
temperature
