摘要
目的:建立30批青蒿样品的UPLC指纹图谱及有机酸类、黄酮类、香豆素类等成分的含量测定方法。方法:采用Agilent Eclipise Plus C18(50 mm×2.1 mm,1.8μm)色谱柱;以0.1%磷酸溶液-甲醇为流动相,梯度洗脱;建立指纹图谱;并对青蒿中有机酸类、黄酮类、香豆素类等9个成分进行含量测定。结果:建立了青蒿UPLC指纹图谱及同时测定多成分含量的方法。9种成分在测定范围内线性关系良好,不同产地的青蒿药材质量存在一定差异。江苏盱眙的青蒿综合评价质量最优。结论:本实验建立的方法准确可靠,可同时测定青蒿中东莨菪内酯、滨蒿内酯、异绿原酸A、异绿原酸B、异绿原酸C、木犀草素、芹菜素、猫眼草黄素、艾黄素的含量。
Objective:To establish the UPLC fingerprint of 30 batches of Artemisia annua and to determine the contents of organic acids,flavonoids and coumarins.Methods:The analysis was carried on an Agilent Eclipise Plus C18(50 mm×2.1 mm,1.8μm)chromatographic column.0.1%phosphoric acid solution-methanol was used as mobile phase for gradient elution to establish the fingerprint.The contents of nine compounds including organic acids,flavonoids and coumarins in Artemisia annua were determined.Results:The methods of UPLC fingerprint and simultaneous determination of multicomponent contents of Artemisia annua were established.The nine components showed good linear relationships in the linear ranges.There were certain differences in quality of Artemisia annua from different regions.The comprehensive evaluation quality of Artemisia annua in Xuyi of Jiangsu was the best.Conclusion:The methods established in this experiment are accurate and reliable,and can simultaneously determine the contents of scopoletin,scoparone,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C,luteolin,apigenin,chrysosplenetin and artemitin.
作者
朱亚莹
吴啟南
段慧芳
乔晶晶
黄志恒
ZHU Ya-ying;WU Qi-nan;DUAN Hui-fang;QIAO Jing-jing;HUANG Zhi-heng(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing 210023,China)
出处
《中药材》
CAS
北大核心
2019年第10期2323-2329,共7页
Journal of Chinese Medicinal Materials
基金
国家中医药管理局专项-国家中药标准化项目(ZYBZH-C-JS-31)
国家现代农业产业技术体系建设专项资金资助(CARS-21)
关键词
青蒿
UPLC
指纹图谱
含量测定
灰色关联度分析
Artemisia annua L.
UPLC
Fingerprint
Content determination
Grey relational analysis
作者简介
朱亚莹(1995-),女,在读硕士研究生,专业方向:中药资源生产与品质评价,E-mail:ayingzyy@163.com;通讯作者:吴啟南,Tel:025-85811010,E-mail:qnwyjs@163.com。
