摘要
用SPF鸡胚将送检的免疫鸡群泄殖腔棉拭样品盲传一代,再用新城疫单抗夹心ELISA对F1代尿囊液进行新城疫病毒(NDV)快速检测,同时用世界动物卫生组织(OIE)推荐的NDV分离及鉴定的方法加以验证。通过对3423份样品的检测,检出阳性样品453份,阴性样品2959份,可疑样品11份,两种方法的阳性符合率为99.3%,阴性符合率为99.7%。对全部阳性样品进行病毒分离,经血凝(HA)和血凝抑制(HI)试验证明均含有新城疫病毒(NDV),再将全部阳性样品分离物进一步作毒力鉴定,结果有强毒力株、中等毒力株及弱毒力株,因此用新城疫单抗夹心ELISA检测免疫鸡群泄殖腔棉拭样品F1代尿囊液新城疫病毒快速、准确率高,呈阳性者不全是NDV强毒力株感染,还有NDV中等毒力株或弱毒力株感染。
Cloacal swab specimens from vaccinated chicken flock were passed o nc e in 10-day-old SPF embryonated chicken eggs. The allantoic fluid was harveste d from 24h to 96h post-innoculation. The sandwich ELISA based on monoclonal ant ibodies was applied to detect Newcastle disease virus using the allantoic fluid harvested. Traditional diagnostic methods recommended by OIE confirmed the NDV v iruses. The results showed that there are 453 positive samples, 2959 negative sa mples and 11 suspicious samples of total 3423 samples detected. The coincidence rate of positive samples is 99.3%and that of negative ones is 99.7%. Further v irulence identification indicated that the velogenic, mesogenic or lentogenic ND V strains existed in these positive samples. So this ELISA method to detect of N ewcastle disease virus is rapid and correct. Positive specimens detected are not all infected by virulent viruses, it might be infected by mesogenic or lentogen ic NDV strains.
出处
《上海交通大学学报（农业科学版）》
2004年第3期286-289,共4页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市农业科学院青年基金项目(未编号)
