摘要
【目的】探讨新城疫-传染性法氏囊病二联灭活疫苗(ND-IBD二联灭活疫苗)与传染性法氏囊病活疫苗(IBD活疫苗)对商品蛋鸡的免疫效果。【方法】选取1日龄商品蛋雏鸡120只分成3个处理组。免疫组分别在雏鸡7日龄时颈部皮下注射0.3 mL IBD活疫苗、0.3 mL ND-IBD二联灭活疫苗,对照组则皮下注射0.3 mL生理盐水。试验期42 d,免疫后每7 d采血分离血清,检测IBDV抗体水平;同时采集法氏囊和脾脏,提取总RNA,检测CD4^+、CD8^+、IL-6的mRNA表达量。然后对各组鸡进行IBDV攻毒保护试验,间隔时间为7 d,攻毒后72 h剖检并对法氏囊和脾脏病变情况进行记录,计算攻毒保护率。【结果】ND-IBD二联灭活苗免疫组IBDV抗体水平显著高于IBD弱毒苗免疫组与对照组;且在免疫后可以有效保护IBDV强毒的感染;ND-IBD二联灭活苗免疫组中法氏囊与脾脏中基因CD4^+、CD8^+、IL-6的m RNA表达量显著高于IBD弱毒苗免疫组与对照组。【结论】ND-IBD二联灭活苗免疫7日龄商品蛋雏鸡的免疫效果优于IBD弱毒苗。
【Objective】 Immunization effects of the inactivated NDV-IBDV combination vaccine and the attenuated live IBDV vaccine on layer chicks were compared. 【Method】 One-hundred-twenty one-day-old commercial layer chicks were randomly divided into 3 groups. In the immunized groups, the 7-d-old chicks were subcutaneously injected 0.3 mL of live IBD vaccine or inactivated NDV-IBDV combination vaccine in the neck, while those in the control group 0.3 mL of physiological saline. During the 42 d of testing, serum samples were collected every 7 d for IBDV antibody, as well as bursal and spleen samples for total RNA extraction to determine the mRNA expressions of CD4^+, CD8^+, and IL-6. A IBDV challenge test was performed on the fowls at a 7 d interval for bursa and spleen lesions by dissection in 72 h to calculate protection rate of the vaccinations. 【Result】 The IBDV antibody in the NDV-IBDV-vaccinated chicks was significantly more than that in the IBDvaccinated or control counterparts. With the immunization by the inactivated NDV-IBDV vaccine, the chicks were effectively protected from the IBDV virulent infection, and the mRNA expressions of CD4^+, CD8^+, and IL-6 in their bursas and spleens significantly higher than those of the birds in either the IBD-vaccinated or the control groups. 【Conclusion】 By combining NDV and IBDV vaccines for the immunization on 7-d-old commercial layer chicks, the disease-prevention effect was better than using IBD vaccine alone.
作者
屈贵蜀
黄瑞玲
星东
彭瑶顺
庄许诺
许丽惠
江兴华
王全溪
QU Guishu;HUANG Ruiling;XING Dong;PENG Yaoshun;ZHUANG Xunuo;XU Lihui;JIANG Xinghua;WANG Quanxi(College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fuzhou Dabeinong Biotechnology Co.,Ltd.,Fuzhou,Fujian 350002,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2020年第7期746-752,共7页
Fujian Journal of Agricultural Sciences
基金
国家星火计划重点项目(2015GA720001)
作者简介
屈贵蜀(1995−),女,硕士研究生,研究方向:预防兽医(E-mail:18104476289@163.com);通信作者:王全溪(1978−),男,博士,副教授,硕士生导师,研究方向:宿主与病原互作(E-mail:wqx608@126.com)
