摘要
本研究探讨建立系统的二甲亚砜 (DMSO)低温保存血小板的制备及质控的技术和方法 ,以保证低温保存血小板的质量。采取的方法 :①DMSO在使用前进行超滤替代消毒 ;②采血后 6小时内对血液进行离心 ,将血袋管道系于套桶上方 ;③以 4 80×g的相对离心力 ,离心加速 9档 ,减速 4档 ;④分离血小板时流速不能太快 ,80 - 10 0ml富含血小板血浆应在 1分钟左右分完 ;在加入DMSO时速度以 1毫升 /分钟为宜 ;加好DMSO后放入 - 80℃冰箱保存 ;临床输注前将血小板置于 38- 4 0℃的循环水浴中 ;⑤融化后进行血小板计数、白细胞和红细胞计数 ,检测HBsAg、抗 HCV、抗 HIV、梅毒特异性抗体 ,测定丙氨酸氨基转移酶 (ALT)并进行细菌培养。结果表明 :共制备14 80 0单位低温保存血小板 ,其中机采血小板 80单位。对其中 30 0单位进行质控。手工分离血小板计数≥ 2 .4×10 10 /单位 ,平均得率在 70 %以上。机采血小板计数≥ 2 .5× 10 11/单位。手工分离血小板红细胞污染数≤ 1× 10 9个/单位 ,白细胞污染数≤ 5× 10 7个 /单位。融化后进行细菌培养 ,无细菌生长 ;ALT均在正常范围内。患者输注后具有明显的止血作用。结论 :本方法制备的手工分离和机采低温保存血小板质量可靠 ,临床应用效果良好。
The purpose of this study was to establish a set of techniques for cryopreservation of platelets with dimethylsulphoxide (DMSO) to insure high quality of cryopreserved platelet. The methods were as following: (1) DMSO was filtered in stead of being sterilized before infusion into the bag with platelets. (2) The whole blood was centrifuged immediately after blood collection and the attached tube was tied on the top of the bucket. (3) The related centrifugal force was 480×g,the accelerating and braking grades of the centrifuge for acceleration and deceleration were 9 and 4 respectively. (4) The flow rate of platelet rich plasma (PRP) could not be too high, 80-100 ml PRP should be harvested at 1 minute or so. The infusion rate of DMSO into the PRP was 1 ml/min. After the infusion of DMSO,the PRP bag must be put into the -80℃ ultra low freezer at once to make the product to be freezed as soon as possible. The cryopreserved platelet should be thawed in the cycling warm water at the temperature of 38-40℃. (5) After thawing of platelet,the platelet,red blood cell and white blood cell were counted,and the bacteria culturing,tests for HBsAg,anti-HCV,anti-HIV,TP and ALT were carried out. The results showed that altogether 14 800 units of cryopreserved platelets were prepared including 80 units collected with blood cell separator,of which quality control was accomplished in 300 units. The manually collected platelet mean count≥2.4×10 10 /unit,while the apheresis platelet count≥2.5×10 11 /unit. The yield was over 70%. The contaminated red and white blood cells were ≤1×10 9 and ≤1×10 7/unit respectively. All the bacteria cultures were negative,while tests for HBsAg,anti-HCV,anti-HIV and TP were negative too. The ALT values were all in normal range. The transfusion of cryopreserved platelets showed obvious effect of haemostasis. In conclusion,the cryopreserved platelets prepared with this method were of high quality and efficaciousness in haemostasis clinically.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第4期519-521,共3页
Journal of Experimental Hematology
关键词
血小板
低温保存血小板
二甲亚砜
质控
输血
platelet
cryopreserved platelet
dimethylsulphoxide
quality control
blood transfusion
