摘要
[目的]探讨丹酚酸B对心脏微血管内皮细胞(CMEC)抗缺氧损伤的保护机制。[方法]基于缺氧预适应的保护机制 ,通过缺氧/复氧的CMEC损伤模型 ,采用分子生物学方法 ,观察蛋白激酶CmRNA表达情况。[结果]丹酚酸B预适应可促进缺氧/复氧损伤的CMEC的蛋白激酶CmRNA表达增强 ,且明显优于缺氧预适应(HPC)。[结论]丹酚酸B预适应与HPC具有相类似的细胞保护效应 ,可增强细胞对随后较长时间缺氧/复氧损伤的耐受性 ,其机制可能是通过增强蛋白激酶CmRNA表达实现的。
Objective] To approach the mechanism of salvianolic acid B in protecting cardiac capillaries endothelial cell(CMEC)against hypoxia.[Methods] Based on the protective mechanism of the advanced adaptation,the expression of protein kinase C-mRNA was observed with molecular biology method,and the protective mechanism of salvianolic acid B on cells was approached.[Result] The expression of C-mRNA was upregulated in salvianolic acid B treated group and better than that in surpassed hypoxia advanced adaptation group(HPC).[Conclusion]Salvianolic acid B advanced adaptation has a similar cellular protective effect as HPC.It can enhance the tolerance longer hypoxia and ambohypoxia.The mechanism may be produced by related to the expressions of the protein kinase C-mRNA.
出处
《天津中医药》
CAS
2004年第4期325-327,共3页
Tianjin Journal of Traditional Chinese Medicine
基金
天津市自然科学基金资助项目 (013612711)
