摘要
在总结多种植原体DNA提取方法的基础上 ,发展了一种提取植原体DNA新方法。用此方法提取的DNA经琼脂糖凝胶电泳检测到大于 15kb的DNA主带 ,基本无DNA碎带 ,不用RNase处理 ,也无RNA干扰 ,OD2 60 / 2 80 值显示产物纯度较高 ,无需任何处理 。
A new method for extracting Phytoplasma DNA from plants was given in the study. DNA extracted by the method had main DNA bands more than 15 kb in molecular length, which can be observed through agarose gel electrophoresis. They do not take RNA without adding RNase. They have a high purity seen by their OD 260/280 value and can be directly used in PCR amplification without any further purification.
出处
《生物技术通报》
CAS
CSCD
2004年第4期44-46,共3页
Biotechnology Bulletin
