摘要
依据已获得的16S rDNA序列,通过BLAST和DNAman软件分析其同源性,找到其保守序列,并设计出NTF1/NTR1、NTF2/NTR1、NTF3/NTR1、NTF1/NTR2、NTF2/NTR2、NTF3/NTR2共5对引物序列,通过优化,筛选出了适宜的PCR检测体系,通过检测验证,该体系能很好地应用于枣疯病植原体的检测。利用优化的检测体系对新疆部分样品进行检测,发现灰枣品种的个别样品携带植原体。
In this paper,five pairs primers(NTF1/NTR1,NTF2/NTR1,NTF3/NTR1,NTF1/NTR2,NTF2/NTR2, NTF3/NTR2)were designed according to the 16 S rDNA conservative sequence,in previous studies it was obtained and analyzed.Through compared and set the different temperature and other conditions of PCR system,the optimum reaction conditions of this PCR detection system was found,and used this method to detect some materials of Xinjiang province,and there were phytoplasma in individual samples of Grey dates.Therefore,this system used in detecting phytoplasma of jujube was feasible,and could provide some formation for relevant study in theory.
出处
《北方园艺》
CAS
北大核心
2011年第11期109-112,共4页
Northern Horticulture
基金
石河子大学重点科技攻关资助项目(gxjs2010-zdgg05-03)
关键词
枣疯病
植原体
检测
jujube infected
phytoplasma
detect
