摘要
目前已明确番茄抗晚疫病R基因表现明显的株龄相关抗性(Age-related Resistance,ARR),但抗晚疫病QTL的抗性规律尚不明确。以携带抗晚疫病基因Ph-3的栽培种番茄(Solanum lycopersicum)‘CLN2037B’和野生种醋栗番茄(S.pimpinellifolium)‘L3708’以及易感病材料栽培种番茄(S.lycopersicum)‘LA2818’为对照,对含有抗晚疫病QTL的多毛番茄(S.habrochaites)‘LA2099’、‘LA1033’和‘LA1777’是否也存在株龄相关抗性进行了分析。结果表明,携带抗晚疫病QTL的3个材料的6叶期和9叶期植株病情级数均比3叶期明显降低,表明QTL抗性与株龄相关。利用乙烯、水杨酸和茉莉酸素合成或缺失的突变体和病毒诱导基因沉默(Virus Induced Gene Silencing,VIGS)技术,初步明确了乙烯和水杨酸参与6叶期Ph-3基因介导的对晚疫病的抗性,而茉莉酸不参与。
Although R genes have been showed significantly age-related resistance(ARR),the mechanism of QTLs involved in this resistance is still unknown. With susceptible material Solanum lycopersicum‘LA2818',resistant material S. lycopersicum‘CLN2037B'and S. pimpinellifolium‘L3708'as control,the age-related resistance of QTL were studied. Except susceptible control LA2818,6-leaf stage and 9-leaf stage plants of S. habrochaites accessions LA2099,LA1033 and LA1777,which contain QTL also showed age-related resistance,were more resistant significantly to late blight disease than 3-leaf stage plants. Thus there is a common defense mechanism in tomato against Phytophthora infestans through ARR. Meanwhile,ethylene,salicylic acid and jasmonic acid synthesis or missing mutants and Virus Induced Gene Silencing(VIGS)technology were combined to study the resistant mechanisms of 6-leaf stage plants harboring Ph-3 gene. The results showed that ethylene and salicylic acid but not jasmonic acid were involved in the resistance to late blight mediated by Ph-3 gene.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第6期1077-1084,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31171975)
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)
农业部园艺作物生物学与种质创制重点实验室项目
