摘要
以提取的苜蓿基因组DNA为模板,通过正交设计,筛选出ISSR-PCR反应体系中最适宜的各组分浓度,即20μL的反应体系中最适添加量分别为2.0 U/μL的Taq DNA聚合酶,0.3 mmol/L的dNTP,1.0 mmol/L的MgCl2,0.1μmol/L的ISSR引物以及30 ng/μL的DNA模板。在此基础上对30份不同苜蓿种质材料进行遗传多样性研究,通过聚类分析研究,将30份不同苜蓿种质材料分为四大类,第一大类为来自加拿大的4个苜蓿品种(系);第二大类为来自美国的12个苜蓿品种(系);第三大类为来自中国和荷兰的12个苜蓿品种(系);第四大类为来自澳大利亚的2个苜蓿品种(系)。
Template DNA used for ISSR was extracted from alfalfa leaf tissue. The orthogonal design was applied to optimize ISSR amplification system. Anoptimal reaction system was established,20 μL reaction system contained 2. 0 U / μL Taq DNA polymerase,0. 3 mmol / L dNTP,1. 0 mmol / L MgCl 2,0. 1 μmol/L ISSR primers and 30 ng / μL DNA template. Genetic diversity studies of Medicago L. germplasms were based on the result of the orthogonal design. And by cluster analysis,30 varieties were divied into four groups,one group was consist of four alfalfa varieties coming from Canada,the second group was composed of twelve varieties coming from America,the third group includes twelve alfalfa varieties coming from China and Netherland,and the last group has two alfalfa varieties coming from Australia.
出处
《华北农学报》
CSCD
北大核心
2013年第S1期45-49,共5页
Acta Agriculturae Boreali-Sinica
基金
北方荒漠草原草地承载力与家畜配置项目(200903060)
农业部牧草种质资源保护项目"牧草温带备份库的维护与备份繁殖入库(2011-10)"
