摘要
为了改善超临界CO2萃取在极性物质方面存在的局限性,在其体系中引入特定表面活性剂和助表面活性剂,考察了它们对超临界CO2萃取人参中皂苷的影响。试验结果发现,表面活性剂和助表面活性剂的加入均可显著提高超临界CO2萃取人参中皂苷的萃取率,其改善效果与它们的种类和加入量有关。在司盘80、吐温80、聚乙二醇辛基苯基醚和琥珀酸二(2-乙基己基)酯磺酸钠4种表面活性剂中,以琥珀酸二(2-乙基己基)酯磺酸钠的改善效果最好,其次是聚乙二醇辛基苯基醚和吐温80,而司盘80最差。在3种助表面活性剂对琥珀酸二(2-乙基己基)酯磺酸钠/超临界CO2反相微乳萃取人参皂苷的改善效果方面,以乙醇效果最好,其次是正戊醇,正丁醇效果最差。在萃取压力32MPa、萃取温度45℃、萃取时间4h和CO2流量2.5L/h的条件下,AOT和乙醇的加入量以0.036g/mL较好,此时人参皂苷的萃取率达15.9%,是没加表面活性剂和助表面活性剂下的13.3倍。
To improve the efficiency of supercritical CO2 extracting polar substances,some certain surfactants and co-surfactants were introduced into supercritical CO2 and their influences on supercritical CO2 extracting ginsenosides from ginseng were explored.The results showed that the surfactants and co-surfactants introduced significantly improved the ginsenoside extraction yield.The degree of yield improvement was correlated with their kinds and adding amount.The order of surfactants that influenced the yield improvement was as follows:bis(2-ethylhexyl)sodium sulfosuccinate>n-octylphenyl polyoxyethylene ether>Tween80>Span80.The order of co-surfactants that influenced the yield improvement was as follows:ethanol>n-pentanol>n-butanol.The optimum amount of AOT and ethanol was 0.036g/mL ethanol under the extraction conditions of extraction pressure of 32 MPa,extraction temperature of 45℃, extraction time of 4h and CO2 flow rate of 2.5L/h,and under these optimum conditions,the ginsenosides yield was 13.3 times of that without surfactants and co-surfactants,reached 15.9%.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2009年第S1期204-207,共4页
Transactions of the Chinese Society of Agricultural Engineering
基金
河南科技大学校青年基金(2007QN064)
