摘要
背景与目的:维生素E琥珀酸酯(vitamin E succinate,VES)是天然维生素E的酯化衍生物,研究已证实其可诱导乳腺癌、前列腺癌、舌癌及肾癌等恶性肿瘤凋亡,却对正常细胞组织没有毒性作用。本实验以VES、紫杉醇单独及联合作用于Her-2过表达乳腺癌细胞,检测各组药物对乳腺癌细胞的诱导凋亡率。方法:免疫细胞化学法检测MDA-MB-453细胞Her-2蛋白表达水平。以VES、紫杉醇单独作用于MDA-MB-453细胞24、48 h,用TUNEL法检测细胞凋亡率,以浓度为10、20 mg/L的VES与浓度为50和100 nmol/L的紫杉醇组合,作用于MDA-MB-453细胞24、48 h,用TUNEL法检测不同组合的细胞凋亡率,比较各种组合对Her-2过表达乳腺癌细胞的诱导凋亡作用。结果:MDA-MB-453细胞Her-2表达率95%CI:63.32%～69.60%;VES与紫杉醇对MDA-MB-453细胞均有诱导凋亡作用,并成剂量和时间依赖关系,其中10 mg/L VES联合100 nmol/L紫杉醇作用于MDA-MB-453细胞48 h对其的诱导凋亡作用最强。结论:VES和紫杉醇对MDA-MB-453细胞均有诱导凋亡作用,两药联合时诱导凋亡作用增强。
Background and purpose:Vitamin E succinate(VES) is the esterification of natural vitamin E derivative.Studies have confirmed that VES could induce the apoptosis of breast cancer,prostate cancer,tongue cancer,kidney cancer,but has no toxicity to normal cells and tissues.This experiment detected the apoptosis rates of Her-2 over-expressing breast cancer cells which were administered with VES and paclitaxel at different dosages,alone or together.Methods:Immunocytochemical method was used to detect Her-2 expression of MDA-MB-453 cells.TUNEL assay was used to detect apoptosis rates of MDA-MB-453 cells treated with VES at the concentration of 10,20 mg/L and paclitaxel of 50 and 100 nmol/L,alone or together,for 24 or 48 h.Then apoptosis action of various combinations was compared.Results:The expression rates of 95% Her-2 were 63.32%-69.60%;VES and paclitaxel both induced apoptosis of MDA-MB-453 cells,and it was dose-and time-dependent.It was strongest in apoptosis at the combination treatment of 10 mg/L VES and 100 nmol/L paclitaxel in MDA-MB-453 cells 48 h later.Conclusion:VES and paclitaxel both can induce the apoptosis of MDA-MB-453 cells.It is stronger when the two drugs are administered together.
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2011年第5期332-336,共5页
China Oncology
基金
黑龙江省教育厅课题(No:11521185)
