摘要
目的采用腹部结扎大鼠胸导管,建立胰淋巴瘀滞动物模型,观察实验动物胰组织结构变化和胰淀肽沉积情况,探讨实验性淋巴瘀滞对胰组织细微结构的影响。方法 20只10月龄SD大鼠随机分为实验(手术)组和对照(假性手术)组;造模后6个月取材,部分胰腺标本行石蜡包埋和组织切片,行常规HE和刚果红染色后光镜观察;部分标本做冰冻切片,进行免疫组织化学染色和光镜观察。结果①实验组大鼠胰组织切片HE染色光镜观察显示,胰腺小叶间隙显著增宽,出现明显的淋巴管扩张、结缔组织增生和脂肪堆积,可见胰岛呈明显淡染。②术后动物刚果红染色切片光镜观察提示,胰腺小叶组织间隙明显增宽,胰岛表现为朱红色刚果红着色现象。③胰淀肽免疫组织化学染色切片光镜观察表明,在术后动物的胰岛及其周围,呈现胰淀肽免疫反应强阳性棕褐色着色反应。结论实验动物胰组织结构的形态学变化提示,胸导管结扎可导致胰淋巴引流障碍,引起胰淋巴管扩张、结缔组织间隙增宽、脂肪堆积以及胰淀肽沉积等,这些结构变化可能影响胰岛的内分泌功能。
Objective In this study,ligated the thoracic duct of rats on the abdomen,and wished to observe whether lymph drainage block animal model can affect pancreatic islet amyloid polypeptide(PIAP)deposit in rats pancreas,and the morphological changes of pancreatic tissue in fine structural level.Methods Twenty adult SD rats at ten months were divided into 2 groups randomly,as experimental(operation)group and control(sham operation)group;The rats were sacrificed at 6 months after operation,the pancreatic tissue paraffin sections were stained with conventional HE and Congo red,some frozen sections were PIAP-immunohistochemistry stained and observed with light microscope.Results ①The conventional HE staining of pancreatic tissue sections in experimental group showed that the pancreatic glandular lobule interstitial space were widen,connective tissue desmoplasia,fatty cumulated,pancreatic islet light coloured.②The Congo red staining sections in experimental group appeared that the pancreatic glandular lobule interstitial space was widen,pancreatic islet stained with cinnabar red colour.③The pancreatic sections in experimental group with PIAP-immunohistochemistry staining displayed that the pancreatic islet and its surrounding tissues were stained strong positive dark brown. Conclusion The morphological changes of rats pancreatic tissue structure after operation indicate that the ligation of thoracic duct may induce pancreatic lymph drainage block,lymphagiectasis, connective tissue interstitial space widen,fatty cumulation,PIAP deposit in rat pancreas;These structural changes may affect the endocrine function of pancreatic islet.
出处
《河北医科大学学报》
CAS
2009年第7期649-651,后插一,共4页
Journal of Hebei Medical University
基金
河北省自然科学基金资助项目(C2007000823)
关键词
胸导管
胰腺
大鼠
thoracic duct
pancreas
rats
作者简介
何海燕(1969-),女,河北深泽人,河北医科大学基础医学院医学博士研究生,从事神经内分泌学基础研究. 王保芝,通讯作者.E-mail:wangbz54@hebmu.edu.cn
