摘要
目的研究铁调素对人成骨细胞(hFOB 1.19)内Ca2+转运的影响。方法将成骨细胞34℃下培养3～4 d至细胞密度为90%,用胰蛋白酶消化后分种于12孔培养板。空白组加双蒸水;实验组加不同浓度铁调素(双蒸水配制),包括H1组(终浓度为10 nmol/L)和H2组(终浓度为50 nmol/L)。干预24 h后用激光共聚焦扫描显微镜(CLSM)检测。结果成骨细胞内钙离子荧光强度空白组为56.38±36.47,H1组为68.01±32.90;H2组为154.06±55.62,3组间的差异有统计学意义(P<0.05)。结论铁调素可促进成骨细胞内Ca2+浓度增加。
0bjective To study the effect of hepcidin concentrations change out of hFOB 1.19 cells on calcium transportation.Methods The hFOB 1.19 was cultured at 34℃ for 3~4 d,being digested with trypsin,then placed in the culture board.D2H2O was added in the control group and hepcidin of different concentration in the experiment group:H1 group(the final concentration was 10 nmol/L) and H2 group(the final concentration is 50 nmol/L).Confocal laser scanning microscope(CLSM) was used in this study 24 hours later.Results...
出处
《苏州大学学报（医学版）》
CAS
北大核心
2008年第1期14-15,37+2,共4页
Suzhou University Journal of Medical Science
基金
江苏省高校自然科学基金资助课题(05KJB320125)
江苏省六大高峰人才项目(07-B-032)
江苏省医学重点人才项目(RC2007100)
