摘要
目的研究产絮凝剂菌株B5的最佳培养基组成和最佳发酵条件。方法通过单因子实验研究不动杆菌B5(Acinetobacter sp.B5)产絮凝剂的最佳碳源、氮源和无机盐,并通过正交设计实验确定菌株B5产絮凝剂的最佳条件。结果培养基的最佳碳源、氮源和无机盐分别为葡萄糖、硫酸铵和CaCl2,葡萄糖含量为2.0%,初始pH值为7.0,培养温度为30℃,摇床转速为160r/min,培养时间为24h,装液量为50ml(250ml三角瓶),接种量为0.2ml时,絮凝率最高(76.85%)。采用在最佳发酵条件下菌株B5的发酵液处理乳品厂废水、生活污水、印染厂废水等废水样品的絮凝率为88.4%～97.2%,其中,以对乳品厂废水的絮凝率最高(97.2%)。可见菌株B5的发酵液对乳品厂废水和生活废水的絮凝效果较好。结论菌株B5产生的絮凝剂可有效地处理各类废水。
Objective To study the optimum component proportion of media and the best fermentation conditions for the flocculatnt-producing strain B5. Methods Through single factor experiment to seek the best carbon sources, nitrogen sources and inorganic salts. Furthermore, through orthogonal test to select the best conditions for producing flocculants by strain B5. Results The best carbon source, nitrogen source and inorganic salt were glucose, (NH4)2SO4 and CaCl2, the content of glucose was 2.0%, the initial pH value of medium was 7.0, the temperature was 30 ℃, agitation rate was 160 r/min and culture time was 24 hours in flakes, 50 ml liquids (250 ml triangular flask), the inoculation volume was 0.2 ml. The flocculants produced by strain B5 under the best fermentation conditions has been furnished for multifarious waste water, the flocculating activities were from 88.4% to 97.2% and reached to the highest on the waste water of dairy farming. Conclusion The flocculants produced by the strain B5 is applicable to the waste water treatment.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第11期883-886,共4页
Journal of Environment and Health
基金
南通大学自然科学基金资助项目(06Z111)
关键词
絮凝
产絮凝剂菌株
最佳培养基
Flocculations
Flocculating-producing strain
Optimum media
作者简介
王兆慧(1968-),女,讲师,硕士研究生,从事环境微生物学教学与研究.
