摘要
目的 探讨miRNA在肝癌中的表达差异,建立其与肝癌预后的关系.方法 提取TCGA数据库中的肝癌样本的miRNA表达量及肝癌临床数据,根据正常组织与肿瘤组织分为两组,通过edgeR算法对比两组数据的miRNA表达量.联合差异miRNA与临床生存时间作生存分析,寻找对肝癌预后产生影响的miRNA.结果 肝癌组织对比正常组织共发现247 个差异表达miRNA (P<0. 01),通过生存分析发现17个差异miRNA与预后相关(P<0. 05),Cox多因素分析结果提示miRNA-30d、miRNA-939、miRNA-188、miRNA-5003与肝癌患者生存时间相关(P<0. 05),差异均有统计学意义.结论 miRNA-30d、miRNA-939、miRNA-188、miRNA-5003在肝肿瘤组织对比正常组织表达差异且肝癌预后相关,对miRNA在肝癌领域的研究具有借鉴价值.
To investigate the differential expression of miRNAs in hepatocellular car-cinoma and to establish a correlation between differential expression of miRNA and prognosis of liver canc-er.Methods The miRNA expression of liver cancer samples and clinical data of liver cancer were extrac-ted from TCGA database.According to the normal tissue and tumor tissue,the miRNA expression levels of the two groups were compared by the edgeR algorithm.Survival analysis was performed by combining differ-ential miRNAs with clinical survival time to find miRNAs that have an impact on the prognosis of liver cancer.Results A total of 247 differentially expressed miRNAs were found in HCC tissues compared with normal tissues(P<0.01).Survival analysis revealed that 17 differential miRNAs were associated with prog-nosis(P<0.05).Cox multivariate analysis revealed miRNA-30d and miRNA-939.miRNA-188 and miR-NA-5003 were associated with survival time of patients with liver cancer(P<0.05),and the difference was statistically significant.Conclusion miRNA-30d,miRNA-939,miRNA-188 and miRNA-5003 are differen-tially expressed in liver tumor tissues compared with normal tissues and prognosis of liver cancer.It is useful for the study of miRNA in the field of liver cancer.
作者
李高
韩涛
张跃
张燕明
李婉君
栾玉婷
杨晓丹
郑振东
Li Gao;Han Tao;Zhang Yue;Zhang Yanming;Li Wanjun;Luan Yuting;Yang Xiaodan;Zheng Zhendong(Shenyang Pharmaceutical Uniuersity,Shenyang 110016,China;Department of Oncology,General Hospital of Northern Theater Command,Shenyang 110016,China;Jinzhou Medical Uniuersity,Jinzhou 121000,China)
出处
《中国临床实用医学》
2019年第4期4-8,共5页
China Clinical Practical Medicine
作者简介
通信作者:郑振东,Email:zhengzhdong@163.com
