摘要
目的观察表没食子儿茶素没食子酸酯(EGCG)对免疫性肝炎小鼠肝损伤的保护作用,探讨Wnt/β-catenin信号通路的作用机制。方法 SPF级C57B6小鼠45只,随机分为3组:对照组(Control)、EGCG药物干预组(EGCG)、免疫性肝炎模型组(AIH),采用ConA诱导性肝损伤法建立AIH模型,EGCG组小鼠予以EGCG(100 mg/kg)灌胃,余组予以等量生理盐水,连续给药28 d,ELISA检测各组谷丙转氨酶(ALT)、谷草转氨酶(AST)变化,苏木精-伊红(HE)染色染色及PAS染色观察各组大鼠肝脏病理学变化及糖原表达,Western blot、qPCR法检测肝脏组织中Wnt/β-catenin信号通路相关因子Wnt1、β-catenin的表达情况。结果 ConA诱导性肝损伤法可以明显导致小鼠肝功能损伤,血浆总ALT、AST表达水平显著升高,病理学可见肝脏组织广泛被破坏,细胞排列紊乱,界线模糊不清,汇管区可见大量条索状胶原纤维增生向肝小叶内延伸,分割破坏肝小叶结构,PAS染色可见糖原表达较低。经EGCG干预后,小鼠肝功能明显得到改善,血浆总ALT、AST表达显著降低,病理学可见肝细胞结构较完整,界线清楚,细胞排列基本有序,部分可见炎性细胞浸润及假小叶形成,PAS染色显著得到改善,且EGCG可以明显下调肝脏组织中Wnt1、β-catenin表达(P<0.05)。结论 EGCG可以明显改善免疫性肝炎小鼠肝功能,减轻肝细胞损伤,其作用机制可能与其介导Wnt/β-catenin信号通路有关。
Objective To observe the protective effect of epigallocatechin gallate(EGCG)on liver injury in mice with immune hepatitis,and to explore the mechanism of Wnt/β-catenin signaling pathway. Methods 45 SPF C57 B6 mice were randomly divided into three groups:The control group(control group),EGCG drug intervention group(EGCG),autoimmune hepatitis(AIH)model group,USES the ConA induced liver injury AIH model is set up,EGCG mice to EGCG(100 mg/kg)to fill the stomach,shall be equivalent physiological saline group,dosing 28 days in a row,ELISA to detect each cereal third transaminase(ALT),aspertate aminotransferase(AST),HE staining and PAS staining to observe each rat liver pathology change,Western blot and qPCR were used to detect the expression of Wnt/β-catenin signaling pathway related factors Wnt1 and β-catenin in liver tissues. Results ConA induced liver injury method can lead to liver injury in mice,significantly increased serum ALT,AST expression levels,widely pathology liver tissues,cells arranged disorder,boundary vague and portal area of article of the funicular collagen fiber hyperplasia extends to hepatic lobule,destroy the structure of hepatic lobule,PAS staining visible expression of glycogen was low. After EGCG intervention,liver function of mice was significantly improved,total plasma ALT and AST expression was significantly reduced,pathology showed that liver cell structure was relatively complete,boundaries were clear,cell arrangement was basically orderly,some inflammatory cell infiltration and pseudolobule formation were observed,and PAS staining was significantly improved. In addition,we found that EGCG can significantly down-regulate the expression of Wnt1 and β-catenin in liver tissues(P < 0.05).Conclusion EGCG can significantly improve liver function,reduce liver cell damage and inhibit liver fibrosis progression in mice with immune hepatitis,and its mechanism may be mediated by Wnt/β-catenin signal pathway by down-regulating the expression of Wnt1 and β-catenin in liver group.
作者
周丹
于洋
安洋
周南
ZHOU Dan;YU Yang;AN Yang;ZHOU Nan(Infectious ward of Tieling Central Hospital,Tieling,Liaoning Province 112000,China)
出处
《解剖学研究》
CAS
2020年第1期38-43,共6页
Anatomy Research
基金
辽宁省自然基金,20180551091.
作者简介
通信作者:周丹,Email:119656739@qq.com
