摘要
NAC蛋白是最大的植物特异性转录因子家族之一,在植物响应生物胁迫和非生物胁迫中具有重要作用。本研究利用二代转录组测序获得牛樟干旱及低温胁迫的转录组数据,并利用本土BLAST从基因组数据中获得牛樟全基因组78个CkNAC转录因子序列,利用分析软件进行保守基序分析。利用转录组分析获得与干旱和低温胁迫相关的CkNAC,进行系统进化和相关基因荧光定量PCR验证。牛樟NAC基因表达谱数据分析发现,有18个基因在受干旱、低温胁迫时表达差异显著,占牛樟NAC家族的23.1%,其中10个基因受干旱胁迫呈高表达水平,占上调基因的55.6%。将14个牛樟NAC转录因子与8个具有抗旱、抗寒NAC蛋白共同构建系统发育进化树,结果显示22个NACs蛋白聚为Ⅰ类和Ⅱ类,参与干旱和低温胁迫的蛋白集中在Ⅰ类。Ⅰ类中CkNAC14、CkNAC23、CkNAC44和CkNAC70分别和参与干旱胁迫的普通小麦(Triticum aestivum)TaNAC67、梭梭(Haloxylon ammodendron)HaNAC38、拟南芥(Arabidopsis thaliana)ANAC072和大豆(Glycine max)GmNAC20聚为一支,其中CkNAC44和CkNAC70与ANAC072和GmNAC20的相似性分别为59.0%和58.2%,推测CkNAC14、CkNAC23、CkNAC44和CkNAC70可能参与调控牛樟在干旱逆境下的反应。利用RT-qPCR技术检测牛樟NAC基因在不同逆境胁迫中的表达变化,结果显示,CkNAC14、CkNAC23、CkNAC44和CkNAC70受干旱胁迫诱导显著上调。本研究基于牛樟高通量转录组数据,探究牛樟NAC转录因子家族生物学特性,为深入研究牛樟NAC基因的非生物胁迫调控提供科学依据。
NAC protein is one of the largest family of plant-specific transcription factors and plays an important role in plant response to biological and abiotic stresses.In this study,the second-generation transcriptome sequencing was used to obtain the transcriptome data of Cinnamomum kanehirai under drought and low temperature stress.Local BLAST was used to obtain 78 CkNAC transcription factor sequences from genomic data,and analysis software was used to analyze the conserved motif.The transcriptome analysis was used to obtain CkNAC associated with drought and low temperature stress,and the phylogenetic and related genes were verified by fluorescence quantitative PCR.The analysis of NAC gene expression profiles showed that 18 genes,accounting for 23.1%of the NAC family of C.kanehirai,had significant expression differences under drought stress and low temperature stress.Among them,10 genes showed high expression level under drought stress,accounting for 55.6%of the up-regulated genes.The phylogenetic tree was constructed by using 14 NAC transcription factors and 8 NAC proteins with drought and cold resistance,according to the results of 22 NACs protein intoⅠ,Ⅱtwo categories,gene involved in drought and low temperature stress concentrated inⅠclass.In theⅠclass,CkNAC14,CkNAC23,CkNAC44 and CkNAC70 were clustered with TaNAC67 of Triticum aestivum,HanAC38 of Haloxylon ammodendron,ANAC072 of Arabidopsis thaliana and GmNAC20 of Glycine max involved in drought stress,CkNAC44 and CkNAC70 had 59.0%and 58.2%similarity with AnAC072 and GmNAC20.It was speculated that CkNAC14,CkNAC23,CkNAC44 and CkNAC70 might be involved in regulating the response of Cinnamomum kanehirai to drought stress.RT-qPCR was used to detect the expression changes of NAC gene in C.kanehirai under different stress conditions,and the results showed that CKNAC14,CKNAC23,CKNAC44 and CKNAC70 were significantly up-regulated by drought stress.Based on the high-throughput transcriptome data of C.kanehirai,this study explored the biological characteristics of the NAC transcription factor family of C.kanehirai,and provided a scientific basis for further study on the regulation of abiotic stress of the NAC gene of C.kanehirai.
作者
张璋
王毅
罗玛妮娅
王丽娟
冯发玉
原晓龙
郑元
Zhang Zhang;Wang Yi;Luo Maniya;Wang Lijuan;Feng Fayu;Yuan Xiaolong;Zheng Yuan(College of Forestry,Southwest Forestry University,Kunming,650224;Key Laboratory of Forest Plant Cultivation and Utilization,Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,Yunnan Academy of Forestry&Grassland Science,Kunming,650201)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2022年第2期363-371,共9页
Genomics and Applied Biology
基金
云南省重大科技专项计划食用菌项目子课题(202002AE320003)
云南省博士后科研基金(188102)
国家自然科学基金项目(31860177)
西南林业大学林学院分子生物学研究团队课题(LKYTD-2020-001)共同资助
关键词
牛樟
NAC转录因子
转录组分析
干旱与低温胁迫
Cinnamomum kanehirai
NAC transcription factors
Transcriptome analysis
Drought and low temperature stress
作者简介
共同通信作者:王毅,dog_608@hotmail.com;共同通信作者:郑元,zhengyuan_001@126.com
