摘要
目的:观察中性粒细胞哮喘(NA)患者诱导痰、外周血中性粒细胞(NEU)髓过氧化物酶(MPO)、白介素8(IL-8)水平、肺功能的特征及其规范治疗后的变化。方法:选取2018年1月至2022年12月本院收治的47例未经规范治疗的慢性持续期哮喘患者,分为NA组(18例)和非NA组(NNA组,29例)。分别于治疗前和治疗1个月后行哮喘控制测试(ACT)评分、肺功能检测,肺功能指标包括第1秒用力呼气容积占预计值百分比(FEV1%)、第1秒用力呼气容积占用力肺活量百分比(FEV1/FVC%)、75%和50%的最大呼气中期流速(MMEF75%/25%)。采用酶联免疫吸附试验(ELISA)法检测外周血和诱导痰MPO、IL-8水平。采用Pearson相关分析法分析各指标的相关性。结果:治疗前,NA组FEV1%、FEV1/FVC%、MMEF75%/25%均低于NNA组(P<0.05),ACT无明显差异(P>0.05),治疗后两组上述指标均较治疗前升高(P<0.05)。治疗前、后NA组诱导痰MPO、IL-8水平和NE%均高于NNA组(P<0.05),治疗后NA组诱导痰IL-8水平、NE%下降(P<0.05),MPO无变化(P>0.05)。治疗前NA组外周血IL-8水平高于NNA组(P<0.05),治疗后外周血IL-8水平显著降低(P<0.05),而MPO、NE%无变化(P>0.05)。两组治疗前痰NE%与FEV1%、MMEF75%/25%呈正相关关系,外周血MPO水平与IL-8呈正相关关系,与ACT评分呈负相关关系(均P<0.05);治疗后,外周血MPO水平与IL-8呈正相关关系(P<0.05)。结论:NA患者痰和外周血MPO、IL-8水平高于NNA患者,肺功能较差;MPO、IL-8与NA肺功能、哮喘控制水平相关,监测MPO、IL-8可指导NA治疗。
Objective:To observe the characteristics of induced sputum,peripheral blood neutrophil(NEU)myeloperoxidase(MPO),interleukin 8(IL-8)levels,lung function and their changes after standardized treatment in patients with neutrophil asthma(NA).Methods:47 patients with chronic persistent asthma admitted to our hospital from January 2018 to December 2022 without standard treatment were selected and divided into NA group(n=18)and non-NA(NNA)group(n=29).Asthma control test(ACT)and lung function test were performed before and one month after treatment,respectively,including percentage of forced expiratory volume in one second to the predicted value(FEV1%),percentage of forced vital capacity in one second to forced vital capacity(FEV1/FVC%),maximum mid expiratory flow rate(MMEF75%/25%).Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of MPO and IL-8 in fasting venous blood and induced sputum and Pearson correlation analysis was used to analyze the correlation of each index.Results:Before treatment,the FEV1%、FEV1/FVC%、MMEF75%/25%in NA group were lower than those in NNA group(P<0.05),there was no significant difference in ACT score(P>0.05),and the above indexes in both groups were higher after treatment(P<0.05).The levels of MPO,IL-8 and the percentage of NEU(NE%)in induced sputum in NA group were significantly higher than those in NNA group before and after treatment(P<0.05),the levels of IL-8 and NE%in induced sputum in NA group decreased After treatment(P<0.05),while MPO remained unchanged(P>0.05).Before treatment,the level of IL-8 in peripheral blood of NA group was higher than that of NNA group(P<0.05),peripheral blood IL-8 levels decreased significantly after treatment(P<0.05),while MPO and NE%remained unchanged(P>0.05).Sputum NE%was positively correlated with FEV1%and MMEF75%/25%before treatment,and peripheral blood MPO level was positively correlated with IL-8,and negatively correlated with ACT score(all P<0.05).After treatment,peripheral blood MPO level was positively correlated with IL-8(P<0.05).Conclusion:Sputum and peripheral blood MPO and IL-8 levels in NA patients are higher than those in NNA patients,and lung function is worse;MPO and IL-8 levels correlate with NA lung function and asthma control levels,and monitoring MPO and IL-8 levels can guide NA treatment.
作者
覃赞梅
黄雪梅
陈晓丽
杨美玲
邓静敏
Qin Zanmei;Huang Xuemei;Chen Xiaoli;Yang Meiling;Deng Jingmin(Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Chengdu Chenghua District Hospital of Traditional Chinese Medicine,Chengdu 610056,China)
出处
《广西医科大学学报》
CAS
2023年第3期487-491,共5页
Journal of Guangxi Medical University
基金
广西医疗卫生适宜技术开发与推广应用项目(No.S2022078)
广西壮族自治区卫生健康委员会自筹经费科研课题(No.Z20191045)
作者简介
通信作者:邓静敏,E-mail:ldyy666@163.com
