摘要
【目的】明确太子参酸腐病病原菌白地霉(Geotrichum candidum)FJAT-32652的生物学特性。【方法】测定白地霉在不同温度、pH值、光照、碳源和氮源培养条件下的菌落直径,以及孢子的致死温度和时长,并用显微镜观察孢子在不同培养时间的萌发情况。【结果】该病原菌生长最适温度为30℃,pH值为6,碳源为麦芽糖、乳糖和葡萄糖,氮源为硫酸铵,不同光照条件对菌丝生长无显著影响,孢子的致死温度及时长为65℃水浴10 min。病原菌孢子培养3 h开始萌发,9 h形成菌丝,繁殖方式为裂殖。【结论】明确了太子参酸腐病病原菌白地霉FJAT-32652的生物学特性,为太子参酸腐病的后续研究提供基础数据。
【Objective】Biological characteristics of the pathogen causing sour rot disease on Pseudostellaria heterophylla were studied.【Method】Diameters of Geotrichum candidum FJAT-32652 colonies on culture medium under varied temperature,pH,light,and carbon and nitrogen sources were measured to determine the optimal growth conditions for the pathogen.Lethal temperature and time of the spores were determined,and spore germination observed under a microscope.【Results】The optimum growth conditions for the pathogen included 30℃,pH 6,maltose,lactose,and glucose for carbon source,and ammonium sulfate for nitrogen source.Light exposure had no significant effect on the mycelial growth.Lethality occurred by submerging the spores in 65℃hot water for 10 min.Spore germination started 3 h after incubation,and mycelia became visible in 9 h.Schizogamy was the reproduction mode of the pathogen.【Conclusion】The biological characteristics of G.candidum were determined for subsequent studies on the sour rot disease of P.heterophylla.
作者
陈燕萍
陈梅春
郑雪芳
刘波
肖荣凤
王阶平
CHEN Yanping;CHEN Meichun;ZHENG Xuefang;LIU Bo;XIAO Rongfeng;WANG Jieping(Agricultural Bio-Resources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2022年第10期1312-1317,共6页
Fujian Journal of Agricultural Sciences
基金
福建省农业科学院自由探索科技创新项目(ZYTS202210)
福建省自然科学基金项目(2022J01486)
关键词
太子参
酸腐病
白地霉
生物学特性
Pseudostellaria heterophylla
sour rot
Geotrichum candidum
biological characteristics
作者简介
陈燕萍(1985-),女,硕士,助理研究员,研究方向:生物技术与生物防治(E-mail:chenyanping071@sina.com);通信作者:肖荣凤(1972-),女,硕士,研究员,研究方向:植物保护(E-mail:xrfxiao@163.com);通信作者:王阶平(1973-),男,博士,研究员,研究方向:微生物生物技术与农业生物药物(E-mail:781063449@qq.com)
