摘要
建立了重铬酸钾褪色光度法测定异烟肼含量的方法,探讨了重铬酸钾褪色光度法测定异烟肼的最佳条件。在硫酸介质中,重铬酸钾能氧化异烟肼,其吸光度随异烟肼质量浓度的增加而减小,且吸光度减小值与异烟肼的质量浓度服从比尔定律,因此通过测定吸光度减小值可间接测定异烟肼的质量。在0.008020~0.04812mg/mL范围内异烟肼质量浓度与吸光度减小值呈良好的线性关系,线性回归方程为ΔA=0.0112+1.5069c(mg/mL),线性相关系数(r)=0.9997。应用于异烟肼片中异烟肼的质量测定,结果与药典法一致。
A new discoloring spectrophotometric determination of isoniazid by potassium dichromate was established.The optimal conditions for the discoloring spectrophotometric determination of isoniazid by potassium bichromate were discussed.In the sulfuric acid medium,potassium bichromate can oxidize isoniazid,and its absorbance decreases with the increase of isoniazid concentration.The value of decreased absorbance is linearly related to the concentration of isoniazid so that the content of isoniazid can be indirectly determined by measuring the value of decreased absorbance.The Beer’s law was obeyed between the mass concentration of isoniazid and the value of decreased absorbance in the range of 0.008020~0.04812 mg/mL while the linear regression equation wasΔA=0.0112+1.5069 c(mg/mL),with a linear correlation coefficient of 0.9997.This proposed method was applied for the determination of the content of isoniazid in isoniazid tablets,and the results agreed well with the pharmacopoeial method.
作者
涂常青
温欣荣
王佳
Tu Changqing(College of Chemistry and Environment,Jiaying University,Meizhou,Guangdong 514015,China)
出处
《化学世界》
CAS
CSCD
2020年第2期116-120,共5页
Chemical World
关键词
异烟肼
重铬酸钾
褪色光度法
isoniazid
potassium bichromate
discoloring spectrophotometry
作者简介
温欣荣,E-mail:198601012@jyu.edu.cn
