摘要
目的探讨miR-124在结肠癌组织中的表达及其对结肠癌细胞生物学行为的影响。方法收集我中心2017年5月-2018年12月收治的24例结肠癌患者的癌组织及癌旁正常组织标本各24例,RT-PCR法检测miR-124的表达。将人结肠癌细胞SW480随机分为空白对照组(未处理组)、miR-124 mimic转染组、miR-124 inhibitor转染组。MTT实验检测细胞增殖能力,Transwell小室法检测细胞侵袭能力。结果与癌旁组织相比,miR-124在结肠癌组织中低表达(P<0.05)。与空白对照组、miR-124 mimic转染组相比,miR-124 inhibitor转染组细胞增殖活性和侵袭能力都明显升高(P均<0.05)。结论miR-124在结肠癌中表达降低并且可以抑制结肠癌细胞的增殖与迁移。miR-124表达异常减少可能是结肠癌发生、发展的因素之一。
Objective To investigate the expression of miR-124 in colon cancer and its effect on the biological behavior of colon cancer cells.Methods From May 2017 to December 2018,24 pairs of specimens of colon cancer and adjacent normal tissues were collected in our hospital.The expression of miR-124 was detected by RT-PCR.Human colon cancer cells SW480 were randomly divided into blank control group(untreated group),miR-124 mimic transfection group and miR-124 inhibitor transfection group.The cell proliferation ability was measured by MTT assay,and the cell invasion ability was detected by Transwell chamber assay.Results Compared with adjacent tissues,miR-124 was down-regulated in colon cancer tissues(P<0.05).Compared with the blank control group and miR-124 mimic transfection group,the cell proliferation activity and invasion ability of miR-124 inhibitor transfection group increased significantly(P<0.05,respectively).Conclusion miR-124 is low expressed in colon cancer and can inhibit the proliferation and migration of colon cancer cells.Abnormal expression of miR-124 may be one of the factors in the development and progression of colon cancer.
作者
周岗
宋鹏
杨靖
ZHOU Gang;SONG Peng;YANG Jing(Department of Medical Oncology,the Second Medical Center,Chinese PLA General Hospital,Beijing 100853,China)
出处
《解放军医学院学报》
CAS
2020年第5期523-525,531,共4页
Academic Journal of Chinese PLA Medical School
作者简介
周岗,男,博士,副主任医师。研究方向:常见消化道肿瘤的相关分子机制。Email:drzg@sohu.com;通信作者:杨靖,男,博士,副主任医师。Email:yangjing_301@126.com
