摘要
目的通过动物造模实验研究,人工智能机械臂模拟一指禅手法,探究不同频率一指禅手法对于脾虚型家兔小肠推进率的影响。方法动物分组:选取普通成年新西兰家兔72只,体质量(2.0±0.5)kg,雌雄各半,分笼饲养并于兔笼外贴标签编为1~72号,随机分为空白组(K组)、模型组(P组)、一指禅推法低频组(A组)、一指禅推法高频组(B组)、抑制剂组1(I-1组)、抑制剂组2(I-2组)。除K组外,其余组均进入造模阶段。造模结束后,除K组和P组外,其余4组均要进行为期10 d的干预治疗。A、B组分别使用机械臂模拟150次/min、200次/min的一指禅手法进行操作,I-1组、I-2组分别使用机械臂模拟150次/min、200次/min的一指禅手法干预后,再进行腹腔注射ML-9(2 mg/kg),连续10 d。完成手法干预后,所有家兔禁食不禁水24 h后处死,采用炭末灌胃法进行小肠推进率的测定,评估不同频率一指禅手法对于小肠推进率的影响。结果(1)K组、A组、B组、I-1组、I-2组小肠推进率均优于P组(P<0.05);(2)A组优于K组(P<0.05),B组与K组无统计学差异(P>0.05);(3)A组小肠推进率优于B组(P<0.05)。结论(1)脾虚造模成功;(2)一指禅手法对脾虚型家兔小肠推进率具有促进作用,且频率150次/min比200次/min促进作用更明显。
Objective Through animal modeling experimental research and artificial intelligence robotic arm simulating the one-finger pushing technique,to explore the influence of it with different frequencies on small intestinal propulsion rates in rabbits with spleen deficiency.Methods Animal groups:72 average adult New Zealand rabbits,weight of(2.0±0.5)kg,male and female in half,reared in separate cages and labelled outside the rabbit cage as No.1-72.These rabbits were randomly divided into blank group(Group K),model group(Group P),one-finger pushing method with low-frequency group(Group A),one-finger pushing method with high frequency group(Group B),inhibitor 1(Group I-1)and inhibitor 2(Group I-2).Except Group K,all the other groups entered the modeling stage.After the modeling,except for Group K and P,the other four groups were required to undergo intervention therapy for 10 days.Group A and B used robotic arm to simulate one-finger pushing technique for 150 times/min and 200 times/min respectively,while Group I-1 and I-2 were intraperitoneally injected with ML-9(2 mg/kg)for 10 consecutive days after one-finger pushing simulated with robotic arm for 150 times/min and 200 times/min respectively.After manipulation intervention,all rabbits were sacrificed 24 h after fasting but with water.Charcoal gavage was used to measure small intestinal propulsion rate,and the influence of different frequency of one-finger pushing on small intestinal propulsion rate was evaluated.Results(1)The small intestine propulsion rates of group K,Group A,Group B,Group I-1 and Group I-2 were better than that of Group P(P<0.05).(2)Group A was superior to Group K(P<0.05),and there was no statistical difference between Group B and Group K(P>0.05).(3)The small intestine propulsion rate of Group A was better than that of Group B(P<0.05).Conclusion(1)Spleen deficiency was successfully modeled.(2)One-finger pushing technique can promote small intestine propulsion rate of rabbits with spleen deficiency,and the promotion effect of frequency 150 times/min is more obvious than that of 200 times/min.
作者
高丽君
王继红
黄泽芳
蔡伟蓝
GAO Lijun;WANG Jihong;HUANG Zefang;CAI Weilan(Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China)
出处
《辽宁中医杂志》
CAS
2022年第5期10-12,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81873395)
关键词
脾虚
一指禅
小肠推进率
频率
人工智能机械臂
spleen deficiency
one-Finger Pushing
small intestinal propulsion rates
frequency
artificial intelligence robotic arm
作者简介
高丽君(1994-),女,广东茂名人,硕士,研究方向:推拿手法量-效关系研究,通元疗法临床应用;通讯作者:王继红(1969-),男,四川广安人,教授,博士研究生导师,博士,研究方向:针灸推拿的临床、教学和科研工作。E-mail:13622882891@163.com。
