摘要
[Objectives]The purpose of this study was to investigate the effects of Pb on rhizosphere soil enzyme activity and chemical constituents of Achyranthes bidentata Blume.[Methods]A.bidentata Blume plants were cultivated in self-made polyvinyl chloride(PVC)pots.The soil was added with different levels of Pb(0,200,400,600,800 and 1000 mg/kg air-dried soil)to investigate the effects of Pb on dry mass,active ingredients(oleanolic acid and ecdysterone)and rhizosphere soil enzyme activity of A.bidentata Blume.[Results]The root dry mass of A.bidentata Blume cultivated in the soil with Pb level above 400 mg/kg significantly reduced.The Pb residues in the A.bidentata Blume.plants growing in the soil with Pb level below 400 mg/kg complied with national standard.The contents of oleanolic acid and ecdysterone in A.bidentata Blume growing in the soil with Pb level above 600 mg/kg declined significantly.At the Pb level of 1000 mg/kg,the activity of urease was inhibited significantly.The activity of phosphatase was inhibited in the presence of Pb in the soil.The activity of sucrase was activated in the soil with Pb level below 400 mg/kg,and was inhibited in the soil with Pb level above 400 mg/kg.[Conclusions]This study has important guiding significance for the reasonable selection of planting base for A.bidentata Blume and the guarantee of its yield and quality.
[Objectives] The purpose of this study was to investigate the effects of Pb on rhizosphere soil enzyme activity and chemical constituents of Achyranthes bidentata Blume. [Methods] A. bidentata Blume plants were cultivated in self-made polyvinyl chloride(PVC) pots. The soil was added with different levels of Pb(0, 200, 400, 600, 800 and 1 000 mg/kg air-dried soil) to investigate the effects of Pb on dry mass, active ingredients(oleanolic acid and ecdysterone) and rhizosphere soil enzyme activity of A. bidentata Blume. [Results] The root dry mass of A. bidentata Blume cultivated in the soil with Pb level above 400 mg/kg significantly reduced. The Pb residues in the A. bidentata Blume. plants growing in the soil with Pb level below 400 mg/kg complied with national standard. The contents of oleanolic acid and ecdysterone in A. bidentata Blume growing in the soil with Pb level above 600 mg/kg declined significantly. At the Pb level of 1 000 mg/kg, the activity of urease was inhibited significantly. The activity of phosphatase was inhibited in the presence of Pb in the soil. The activity of sucrase was activated in the soil with Pb level below 400 mg/kg, and was inhibited in the soil with Pb level above 400 mg/kg. [Conclusions] This study has important guiding significance for the reasonable selection of planting base for A. bidentata Blume and the guarantee of its yield and quality.
基金
Supported by Project of the Education Department of Henan Province(19B360002)
Key Discipline Construction Project of Henan University of Animal Husbandry and Economy for Pharmacognosy of Veterinary Herbal Medicine(41000003).
作者简介
Corresponding author:Xinmin WANG,E-mail:wangxinmindr@163.com
