摘要
【目的】探讨白细胞介素-29(interleukin-29,IL-29)对结肠癌细胞增殖、迁移、侵袭的影响及分子机制。【方法】用浓度分别为10、100、1000 ng/ml的重组人IL-29处理SW1116细胞作为不同浓度的IL-29处理组,以未经任何处理的细胞作为control组;将pcDNA3.1、pcDNA3.1-蛋白激酶Bα(protein kinase Bα,AKT1)转染至SW1116细胞中再用1000 ng/ml的IL-29处理作为IL-291000 ng/ml+pcDNA3.1组、IL-291000 ng/ml+pcDNA3.1-AKT1组。免疫组化染色检测结肠癌组织和癌旁组织中IL-29的表达;四甲基偶氮唑盐比色法(MTT)测定细胞存活率;蛋白质印迹(Western blotting)检测细胞核增殖抗原标记物(Ki67)、上皮钙粘蛋白(E-cadhexin)、基质金属蛋白酶2(matrix metallopeptidase 2,MMP-2)、AKT1蛋白表达;Transwell检测细胞迁移和侵袭。【结果】与癌旁组织相比,结肠癌组织中IL-29阳性表达率显著降低(P<0.001);与control组比较,100、1000 ng/ml浓度IL-29处理的SW1116细胞中细胞存活率显著降低,Ki67表达水平显著降低,E-cadherin表达水平显著升高,MMP-2表达水平显著降低,细胞迁移、侵袭数显著降低,AKT1表达水平显著降低(P<0.001);过表达AKT1逆转IL-29对细胞SW1116增殖、迁移、侵袭的抑制作用。【结论】一定浓度的IL-29可抑制结肠癌细胞的增殖、迁移和侵袭,其机制可能与AKT1有关。
【Objective】To explore the effect of interleukin-29(IL-29)on the proliferation,migration and invasion of colon cancer cells and its molecular mechanism.【Methods】SW1116 cells were treated with recombinant human IL-29 at the concentrations of 10 ng/ml,100 ng/ml,and 1000 ng/ml as treatment groups of different concentrations of IL-29,and cells without any treatment were used as control group.pcDNA3.1 and pcDNA3.1-AKT1 were transfected into SW1116 cells,and then the cells were treated with 1000 ng/ml IL-29 as IL-291000 ng/ml+pcDNA3.1 group and IL-291000 ng/ml+pcDNA3.1-AKT1 group.Immunohistochemical staining was used to detect the expression of IL-29 in colon cancer tissues and paracancerous tissues.Cell viability was determined by MTT assay.Western blotting was used to detect the expression of nuclear-associated antigen Ki-67,epithelial cadherin(E-cadhexin),matrix metalloproteinase 2(MMP-2),phosphorylated protein kinase Bα(p-AKT1).Transwell was used to detect cell migration and invasion.【Results】The positive expression rate of IL-29 in colon cancer tissues was significantly lower than that in adjacent tissues(P<0.05).Compared with the control group,the survival rate was significantly decreased in 100 ng/ml and 1000 ng/ml IL-29-treated SW1116 cells;the expression of Ki67 was significantly decreased;the expression of E-cadherin was significantly increased;the expression of MMP-2 was significantly decreased;the number of cell migration and invasion was significantly decreased and the expression of AKT1 was significantly decreased(P<0.05).The overexpression of AKT1 reversed the inhibitory effect of IL-29 on proliferation,migration and invasion of SW1116 cells.【Conclusion】A certain concentration of IL-29 can inhibit the proliferation,migration and invasion of colon cancer cells,and its mechanism may be related to AKT1.
作者
李渊
黄少辉
李淑婧
LI Yuan;HUANG Shao-hui;LI Shu-jing(Department of the 2nd Surgical,Gansu Corps Hospital of PAP,Lanzhou 730050,China)
出处
《武警后勤学院学报（医学版）》
CAS
2020年第1期11-15,共5页
Journal of Logistics University of PAP（Medical Sciences）
作者简介
李渊,本科,主治医师,主要从事结直肠方面的研究。
