摘要
目的研究葛根地榆紫草汤组方对溃疡性结肠炎(UC)小鼠的保护作用。方法按照体重将60只健康小鼠随机分成6组:空白组(10只),模型组(10只),柳氮磺胺吡啶组(10只),葛根地榆紫草汤组方低、中、高3个剂量组(各10只)。除空白组外其余小鼠结肠注入三硝基苯磺酸(TNBS)和乙酸制备UC小鼠模型。造模后24 h开始给药,每日2次,连续给药14 d。观察小鼠疾病活动指数,以酶联免疫吸附法测定血清中白介素-1(IL-1)、白介素-6(IL-6)、白介素-10(IL-10)及环氧酶2(COX-2)的含量。结果与模型组相比,葛根地榆紫草汤组方中、高剂量组小鼠疾病活动指数、结肠病变及组织学评分显著降低(P<0.05);葛根地榆紫草汤组方低、中、高各剂量组促炎因子IL-1、IL-6及COX-2含量均显著降低(P<0.05),而抗炎因子IL-10含量显著升高(P<0.05)。结论葛根地榆紫草汤组方对UC小鼠的症状有一定的缓解作用,其机制可能与影响IL-1、IL-6、IL-10、COX-2的表达有关。
Objective To study the protective effect and mechanism of Gegendiyuzicao soup on ulcerative colitis mouse.Methods The 60 mouse were randomly divided into six groups(n=10):blank group(group A),UC model group(group B),SASP group(group C),low,middle and high dose intervention groups(group D,E,F).The model of ulcerative colitis was established by injected dinitrochlorobenzene(DNCB)and acetic acid into colon of mouse except for the blank group,and then drugs was given twice daily for 14 days.The index of disease activity was observed.The levels of interleukin-1(IL-1),interleukin-6(IL-6),interleukin-10(IL-10)and cyclooxygenase-2(COX-2)in the serum were measured by ELISA.Results Compared with the model group,the index of disease activity,colonic lesions and histological score of mouse in the middle and high dose groups were reduced significantly(P<0.05).The content of pro-inflammatory factor IL-1,IL-6 and COX-2 decreased significantly in the low,middle and high dose groups(P<0.05),while the content of antiinflammatory factor IL-10 increased significantly(P<0.05).Conclusion The Gegendiyuzicao soup had a certain effect on the symptoms of UC mouse.The mechanism might be related to influencing the expression of IL-1,IL-6,IL-10 and COX-2.
作者
姜爱雯
杜佩珊
刘云宁
汤建华
梁江
张鹤鸣
JIANG Ai wen;DU Pei shan;LIU Yun ning;TANG Jian hua;LIANG Jiang;ZHANG He ming(Deptartment of Pharmacy,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000;The First Hospital of Zhangjiakou,Zhangjiakou,Hebei 075000,China)
出处
《热带医学杂志》
CAS
2019年第12期1477-1479,1484,1454,共5页
Journal of Tropical Medicine
基金
张家口市科技计划项目(1521025D).
作者简介
姜爱雯(1981-),男,硕士,主管药师,从事临床药学工作,E mail:lonyboy81@126.com
