摘要
目的:探讨益气活血养阴汤辅助治疗糖尿病视网膜病变(DR)疗效及对外周血单个核细胞Kelch样ECH相关蛋白(Keap1)-转录因子核因子红细胞2相关因子2(Nrf2)/抗氧化反应元件(ARE)通路的影响。方法:选择我院2022年7月-2023年7月DR患者120例,将其随机分为观察组与对照组,各60例。对照组口服羟苯磺酸钙分散片,观察组在对照组基础上口服益气活血养阴汤。2组治疗周期12周。统计2组治疗总有效率。比较2组治疗前后中医证候积分,黄斑中心凹厚度和视力,眼底病变,Keap1和Nrf2蛋白表达。结果:观察组总有效率高于对照组(P<0.05)。治疗后,2组DR患者视物昏花和目睛干涩积分较治疗前降低(P<0.05);治疗后,观察组DR患者视物昏花和目睛干涩积分低于对照组(P<0.05)。治疗后,2组DR患者黄斑中心凹厚度较治疗前降低,而视力较治疗前上升(P<0.05);治疗后,观察组DR患者黄斑中心凹厚度低于对照组,而视力高于对照组(P<0.05)。治疗后,2组DR患者出血斑面积、视野灰度值和血管瘤体积较治疗前降低(P<0.05);且观察组低于对照组(P<0.05)。治疗后,2组DR患者Keap1蛋白灰度较治疗前降低,而Nrf2蛋白灰度值较治疗前上升(P<0.05);治疗后,观察组DR患者Keap1蛋白灰度低于对照组,而Nrf2蛋白灰度值高于对照组(P<0.05)。结论:益气活血养阴汤辅助治疗DR患者疗效良好,其机制可能与调控外周血单个核细胞Keap1-Nrf2/ARE通路有关。
Objective:To investigate the efficacy of Yiqi Huoxue Yangyin Decoction in the adjuvant treatment of diabetes retinopathy(DR)and its effect on the Kelch like ECH related protein(Keap1)-transcription factor nuclear factor erythrocyte 2 related factor 2(Nrf2)/antioxidant response element(ARE)pathway of peripheral blood mononuclear cells.Methods:120 DR patients in our hospital from July 2022 to July 2023 divided observation group and control group,each 60 patients.The control group received oral administration of calcium hydroxybenzenesulfonate dispersible tablets,while the observation group received oral administration of Yiqi Huoxue Yangyin Decoction basis of control group.Two treatment cycles of 12 weeks.Calculate the total effective rate of two treatment groups.Compare the traditional Chinese medicine syndrome scores,macular fovea thickness and visual acuity,fundus lesions,and Keap1 and Nrf2 protein expression between two groups.Results:The observation group effective higher control group(P<0.05).After treatment,two groups of DR patients scores of blurred vision and dry eyes decreased compared before treatment(P<0.05);observation group of DR patients scores of blurred vision and dry eyes lower control group(P<0.05).After treatment,two groups of DR patients thickness of the macular fovea decreased compared before treatment,while their vision increased compared before treatment(P<0.05);observation group macular fovea thickness of DR patients lower control group,while their visual acuity higher control group(P<0.05).After treatment,two groups of DR patients area of bleeding spots,grayscale values of visual field,and volume of vascular tumors decreased compared before treatment(P<0.05);observation group bleeding spot area,visual field grayscale value,and vascular tumor volume of DR patients lower control group(P<0.05).After treatment,two groups of DR patients grayscale of Keap1 protein decreased compared before treatment,while the grayscale value of Nrf2 protein increased compared before treatment(P<0.05);observation group of DR patients Keap1 protein grayscale lower control group,while the Nrf2 protein grayscale value higher control group(P<0.05).Conclusion:Yiqi Huoxue Yangyin Decoction has a good therapeutic effect in assisting the treatment of DR patients,and its mechanism may be related to the regulation of the Keap1-Nrf2/ARE pathway in peripheral blood mononuclear cells.
作者
李君卿
陶雯璇
张元坤
张京红
Li Junqing;Tao Wenxuan;Zhang Yuankun;Zhang Jinghong(Zhangjiakou Fourth Hospital,Zhangjiakou 075000,China)
出处
《生命科学仪器》
2023年第6期182-185,共4页
Life Science Instruments
基金
河北省中医药管理局中医药类科研指令性计划课题(2021444)
