摘要
目的:通过动脉硬化ApoE^(-/-)小鼠实验,探讨消癥通络法调节PPARγ相关通路干预动脉粥样硬化的作用机制。方法:选取25只C57BL/6小鼠作为空白组,68只ApoE^(-/-)小鼠随机分为模型组(24只)、中药组(22只)、对照组(22只),12周后观察小鼠状态,主动脉HE染色、油红O染色观察血管病理变化、斑块面积,并通过免疫组化、Western blot观察血管ox-LDL、PPARγ、CD36、ABCA1蛋白表达情况。结果:(1)一般情况:空白组小鼠状态良好,皮色光亮,活跃,饮食饮水正常;模型组大鼠状态较差,皮毛油腻、晦暗,神情萎靡,饮食稍差,饮水正常;与模型组相比较,中药组及对照组给药后均有不同程度改善。(2)HE染色及油红O病理图片显示:空白组主动脉未见AS斑块;与空白组相比,模型组AS斑块面积显著增大;与模型组相比,对照组、中药组AS斑块面积明显减小。(3)免疫组化法及Western blot检测小鼠主动脉ox-LDL、PPARγ、CD36、ABCA1蛋白表达:与空白组比较,模型组小鼠主动脉PPARγ、ABCA1蛋白表达显著降低,ox-LDL、CD36蛋白表达显著升高(P<0.01);与模型组相比,中药组小鼠主动脉PPARγ、ABCA1蛋白表达明显升高(P<0.05),CD36明显降低(P<0.01),ox-LDL降低,但无统计学意义(P>0.05)。结论:消癥通络法能够抑制PPARγ介导的CD36信号通路,促进ABCA1信号通路,从而达到改善血管内皮炎症反应,抑制巨噬细胞泡沫化,延缓AS进展的目的。
Objective:To explore the mechanism of the Method of eliminating symptoms and dredging collaterals regulating PPARγrelated pathway in the intervention of atherosclerosis through the experiment of arteriosclerosis ApoE^(-/-)mice.Methods:25 C57BL/6 mice were selected as the blank group,and 68 ApoE^(-/-)mice were randomly divided into model group(24),traditional Chinese medicine group(22)and control group(22).After 12 weeks,the state of mice was observed,the pathological changes and plaque area of aorta were observed by HE staining and oil red O staining,and the expressions of ox-LDL,PPARγ,CD36 and ABCA1 proteins were observed by immunohistochemistry.Results:①General conditions:The mice in the blank group were in good condition,bright and active skin color,normal diet and drinking water;The rats in the model group were in poor condition,with greasy and dark fur,depressed expression,poor diet and normal drinking water;Compared with the model group,the traditional Chinese medicine group and the control group improved in varying degrees.②The results of HE staining and Oil Red O pathological pictures:No atherosclerotic plaque was found in the aorta of the normal group.Compared with the normal group,the plaque area of the model group increased significantly.Compared with the model group,the plaque area of the control group and the traditional Chinese medicine group decreased significantly.③The results of immunohistochemical detection and Western blot of ox-LDL,PPARγ,CD36 and ABCA1 protein in mouse aorta:Compared with the normal group,the positive areas of PPARγand ABCA1 in the aorta of the model group decreased significantly,and the positive areas of ox-LDL and CD36 increased significantly(P<0.01);Compared with the model group,the protein expression of PPARγand ABCA1 in aorta of mice in traditional Chinese medicine group increased significantly(P<0.05),CD36 decreased significantly(P<0.01),and ox-LDL decreased,but there was no significant difference(P>0.05).Conclusion:the Method of eliminating symptoms and dredging collaterals can inhibit PPARγmediated CD36 signaling pathway and promote ABCA1 signaling pathway,so as to improve vascular endothelial inflammatory response,inhibit macrophage foam and delay atherosclerosis progression.
作者
张欣
葛建立
苏坤
张敏妹
张静
何建明
马云龙
孙云朝
楚信强
ZHANG Xin;GE Jianli;SU Kun(Chinese medicine hospital of Hebei Province,Shijiazhuang Hebei050001,China)
出处
《四川中医》
2023年第10期63-68,共6页
Journal of Sichuan of Traditional Chinese Medicine
基金
河北省科技厅科技支撑计划项目(编号:19277753D)
河北省中医药管理局(编号:2018046)
河北省中医药管理局(编号:2019066)
河北省中医药管理局(编号:2019004)
作者简介
张欣,主治医师,硕士研究生,主要研究方向:周围血管疾病的临床与研究;通讯作者:葛建立,主任医师,教授,硕士研究生,主要研究方向:周围血管疾病的临床与研究,E-mail:360330013@qq.com。
