摘要
为明确美拉德反应产物挥发性成分及不同前处理方法对挥发性成分种类及含量的影响,以果糖和3种氨基酸的美拉德反应产物为研究对象,通过同时蒸馏萃取、溶剂萃取、固相微萃取3种萃取方式,对美拉德反应产物进行前处理,利用GC-MS技术对挥发性成分进行分析鉴定。结果表明:(1)3种萃取方式检测出的挥发性成分共有75种。同时蒸馏萃取法检测出52种香味物质,对酯类和含氮杂环类有较好的萃取效果;溶剂萃取法检测出20种香味物质,对酮类物质、含氧杂环类及含氮杂环物质有较好的萃取效果;固相微萃取法共检测出27种香味物质,对酯类及烷烃类物质有较好的萃取效果。(2)利用主成分分析法,得出的主要挥发性成分有甲基吡嗪、糠醛、糠醇、戊酸乙酯、2,6-二甲基吡嗪、5-甲基糠醇、5-甲基糠醛、己酸乙酯、4-羟基-2,5-二甲基-3(2H)呋喃酮、2,3-二氢-3,5-二羟基-6-甲基-4H-吡喃-4-酮等。
In order to determine the volatile components of Maillard reaction products and clarify the effect of different pretreatment methods on the types and contents of volatile components,fructose and three amino acids which are the Maillard reaction products(MRP)were selected as study subjects.Simultaneous distillation extraction,solvent extraction and solid phase microextraction were used to pretreat MRPs,and the volatile components were analyzed and identified by GC-MS.The results showed that there were 75 volatile components detected by the three extraction methods.52 kinds of aroma substances had been detected by using SDE method.And SDE method had good extraction effects on esters and nitrogen-containing heterocyclics.20 kinds of aroma substances were detected by LLE method,and it had good extraction effects on ketones,oxygen-containing heterocyclics and nitrogencontaining heterocyclic substances.27 kinds of aroma substances had been detected by SPME method and it had good extraction effects on esters and alkanes.By using principal component analysis,the main volatile components that has been detected were methylpyrazine,furfural,decyl alcohol,ethyl valerate,2,6-dimethylpyrazine,5-methylnonanol,5-methylfurfural,ethyl hexanoate,4-hydroxy-2,5-dimethyl-3(2 H)furanone,2,3-dihydro-3,5-dihydroxy-6-methyl-4 H-pyran-4-Ketone and so on.
作者
赵琪
李瑞丽
冯衍闯
苏加坤
徐达
蔡继宝
张峻松
ZHAO Qi;LI Ruili;FENG Yanchuang;SU Jiakun;XU Da;CAI Jibao;ZHANG Junsong(Colloge of Food and Bioengineering,Zhengzhou University of light Industry,Zhengzhou 450001,China;China Tobacco Jiangxi Industrial Co.,Ltd.,Nanchang 330096,China)
出处
《食品科技》
CAS
北大核心
2020年第1期301-306,共6页
Food Science and Technology
基金
江西中烟工业有限责任公司科技项目(赣烟工科计2017-2)。
关键词
美拉德反应产物
同时蒸馏萃取
溶剂萃取
固相微萃取
GC-MS
主成分分析
Maillard reaction product
simultaneous distillation extraction
solvent extraction
solid phase microextraction
GC-MS
principal component analysis
作者简介
赵琪(1996—),女,硕士研究生,研究方向为烟草化学与香精香料。;通信作者:张峻松
