摘要
目的:通过回收试验评估Beckman AU5800全自动生化分析仪测定胸水、腹水、脑脊液、穿剌液等标本类型多项生化检测项目的基质效应。方法:收集临床新鲜胸水、腹水、引流液和脑脊液,使用Beckman AU5800全自动生化分析仪检测系统测定乳酸脱氢酶(LDH)、天冬氨酸氨基转移酶(AST)、淀粉酶(AMY)、总胆固醇(CHOL)、总蛋白(TP)、白蛋白(ALB)、葡萄糖(GLU)等项目。通过回收试验计算回收率评估各种体液的基质偏差大小。结果:在本研究中,使用Beckman AU5800全自动生化分析仪测定血清样本的检测程序对新鲜体液标本以上各项测定的回收率均在(100%±10%)范围内,为临床可接受范围之内。结论:使用Beckman AU5800全自动生化分析仪测定血清样本的检测程序测定新鲜体液标本的基质效应在可接受偏差之内,测定结果准确可靠。
Objective:To evaluate the matrix effect of body fluid specimens(including pleura,ascites,drainage and cerebrospinal)by performing recovery study on Beckman AU5800 system.Method:Collecting clinical body fluid specimens(including pleura,ascites,drainage and cerebrospinal),and then all the chemistry analytes,including lactate dehydrogenase(LDH),aspartate aminotransferase(AST),amylase(AMY),total cholesterol(CHOL),total protein(TP),albumin(ALB)and glucose(GLU),were tested on Beckman AU5800 system.The rate of recovery study(spiking with high concentration serum)was calculated to evaluate the matrix deviation of the body fluids.Result:The recovery rates of the majority of body fluid specimens were within the acceptable limit of(100%±10%).Conclusion:The body fluid matrix interferences for commonly requested chemistry analytes in body fluid specimens are acceptable on Beckman AU5800 system.
作者
石伍和
刘凤
张科
张德太
SHI Wuhe;LIU Feng;ZHANG Ke;ZHANG Detai(Department of Clinical Laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan,430022,China)
出处
《临床血液学杂志（输血与检验）》
CAS
2020年第1期109-111,共3页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
关键词
体液
基质效应
回收试验
body fluid
matrix effect
recovery study
作者简介
通信作者:张德太,E-mail:Detaizhangwh@163.com
