摘要
目的探究中老年痰湿质与平和质人群肠道菌群样品在物种分类、丰度及多样性等方面的差异,找出中老年痰湿质人群肠道的特征功能菌群,以期为痰湿质与代谢性疾病相关性研究提供肠道微生物层面的依据。方法选择于2017年5月—12月在浙江医院中医内科就诊或体检中心行健康体检的人群为研究样本,通过中医体质辨识软件结合专业人员辨证,纳入痰湿质与平和质各30人,采集粪便样品并对其总RNA进行提纯,用Miseq测序仪进行基于16SrRNA的生物多样性测序,利用R(v3.1.1)软件对样品的物种分类、丰度、多样性及组间差异等进行统计分析。结果①各样品间菌群构成及相对丰度均存在差异,但主要构成菌种均为拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)及放线菌门(Actinobacteria)。②痰湿质组的observed species指数、chao指数、ACE指数及shannon指数均小于平和质组(均P<0.05),simpson指数大于平和质组(P<0.05)。③痰湿质组拟杆菌门(38.439±13.545)相对丰度低于平和质组(46.669±12.984),厚壁菌门(52.802±13.861)相对丰度高于平和质组(45.742±12.990),差异有统计学意义(均P<0.05)。结论痰湿质人群肠道菌群的丰度及多样性均显著降低;拟杆菌门相对丰度降低及厚壁菌门相对丰度升高为痰湿质人群肠道菌群的重要结构特征。
Objective To explore the differences in species classification,abundance and diversity of intestinal flora samples between middle-aged and elderly people with phlegm-dampness and balanced constitution,and to find out the characteristic functional intestinal flora in middle-aged and elderly people with phlegm-dampness constitution,so as to provide the basis for the study of the relationship between phlegm-dampness constitution and metabolic diseases from the respect of microorganism.Methods People who went to department of TCM for diagnosis and treatment or medical examination center for physical examination in our hospital from May to December 2017 was selected as the study samples,and totally 30 persons with phlegm-dampness constitution and 30 persons with balanced constitution were included through the combination of TCM constitution identification software and professional syndrome differentiation.The fecal samples were collected and the total RNA was purified.The biodiversity sequencing was carried out by Miseq sequencing technology based on 16 SrRNA,and the species classification,abundance,diversity and differences between the two groups were analyzed by R(v3.1.1)software.Results①The composition and relative abundance of intestinal flora were different among samples,but the main floras were Bacteroidetes,Firmicutes,Proteobacteria and Actinobacteria.②The observed species,chao,ACE and shannon index of phlegm-dampness group were all smaller than those of balanced group(all P<0.05),and the simpson index was larger than that of balanced group(P<0.05).③The relative abundance of Bacteroidetes(38.439±13.545)in phlegm-dampness group was lower than that in balanced group(46.669±12.984),while the relative abundance of Firmicutes(52.802±13.861)was higher than that in balanced group(45.742±12.990),and the difference was statistically significant(all P<0.05).Conclusion The species abundance and diversity of intestinal flora in people with phlegm-dampness constitution are significantly reduced.The relative abundance of Bacteroidetes decreasing and Firmicutes increasing are important structural features of intestinal flora in people with phlegm-dampness constitution.
作者
马晔琳
钱晓玲
杨敏春
MA Ye-lin;QIAN Xiao-ling;YANG Min-chun(Department of TCM,Zhejiang Hospital,Hangzhou,Zhejiang 310013,China)
出处
《中华全科医学》
2020年第1期113-118,共6页
Chinese Journal of General Practice
基金
国家自然科学基金项目(81804163)
浙江省中医药科技计划项目(2017ZQ003,2018ZB006,2019ZB003).
关键词
中老年
痰湿质
平和质
肠道菌群
结构特征
Middle-aged and elderly people
Phlegm-dampness constitution
Balanced constitution
Intestinal flora
Structure characteristics
作者简介
通信作者:杨敏春,E-mail:zjyyymc@163.com
