摘要
目的通过高效液相色谱法(High Performance Liquid Chromatography,HPLC)探索香附中α-香附酮的提取方式和含量的测定方法,达到方法准确可靠简便易操作的目的。方法采用高效液相色谱法。色谱柱为Agilent Eclipse Plus,十八烷基硅烷键合硅胶色谱柱,150×4.6 mm,5μm,流动相为:甲醇—水(70:30),紫外-可见分光检测器;流速为1.0 mL/min;检测波长为240 nm;柱温为35℃。结果香附在0.02314-0.46287 mg/mL线性范围内呈良好线性关系,R2=1,平均回收率99.84%,RSD(Relative Standard Deviation)=0.09%。结论该方法操作简便,灵敏度高,重复性好,准确、可靠,可用于测定香附α-香附酮的含量,并有效控制香附的质量。
Objective By means of High Performance Liquid Chromatography(HPLC),the extraction method and content determination method ofα-cyperone were explored to achieve the purpose of accuracy,reliability,convenience and easy operation.Methods High performance liquid chromatography was used.The chromatography was performed on Agilent Eclipse Plus(150×4.6 mm,5μm)silica gel column with mobile phase consisted of methanol-water(70:30)and UVVIS spectrometer.The flow rate was 1.0 mL/min.The detection wavelength was 240 nm.The column temperature was 35℃.Results The results showed a good linear relationship in the linear range of 0.02314-0.46287 mg/mL with R2=1,the average recovery rate of 99.84%and relative standard deviation(RSD)=0.09%.Conclusion The method has the advantages of simple operation,high sensitivity,good repeatability,accuracy and reliability,and can be used to determine the content ofα-cyperone in order to control the quality of cyperone effectively.
作者
何桂英
邓竹君
郑好
涂雨佳
岳玲燕
罗恩莉
HE Gui-Ying;DENG Zhu-Jun;ZHEN Hao;TU Yu-Jia;YUE Ling-Yan;LUO En-Li(Sichuan Mianyang Pharmaceutical Co.,Ltd.,Taiji Group,Mianyang 621000,China)
出处
《实验室检测》
2024年第5期18-21,共4页
Laboratory Testing
关键词
香附
Α-香附酮
紫外-可见分光检测器
高效液相色谱法
Cyperi Rhizoma
alpha-cyperone
ultraviolet-visible spectroscopy detector
high performance liquid chromatography
作者简介
通信作者:何桂英,副主任中药师,经理,研究方向为中药材鉴定、中成药生产管理、中成药质量管理,药物警戒等。E-mail:457315191@qq.com
