摘要
目的研究侧柏Platycladus orientalis叶的化学成分及抗氧化和α-葡萄糖苷酶抑制活性。方法采用硅胶、MCI、聚酰胺及制备液相色谱分离纯化并用波谱技术进行结构鉴定。采用DPPH和ABTS法进行抗氧化活性研究,pNPG法进行α-葡萄糖苷酶抑制活性研究。结果从侧柏叶80%乙醇提取物中分离得到了11个化合物,分别鉴定为4-O-(1′,3′-二羟基丙基-2′-)-二氢松柏醇9-O-β-D-葡萄糖苷(1)、杨梅苷(2)、5,8,3′,4′-四羟基黄酮7-O-β-D-木糖苷(3)、isomassonianoside B(4)、(-)-异落叶松脂素9′-O-β-D-葡萄糖苷(5)、(7R,8S,7′S,8′R)-4,9,4′,7′-tetrahydroxy-3,3′-dimethoxy-7,9′-epoxylignan4-O-β-D-glucopyranoside(6)、柳杉酚(7)、桃柁酚(8)、5,6-dehydrosugiol methyl ether(9)、isopimara-8,15-dien-7-one(10)和α-L-鼠李糖乙醇苷(11)。结论化合物1为新化合物,命名为侧柏苷A;已知化合物中,7个化合物(4~7、9~11)为首次从侧柏植物得到,6个(4~6、9~11)为首次从侧柏亚科植物中得到,4个(5、6、10、11)为首次从柏科植物中得到。化合物2~6显示出了一定的抗氧化活性;2和3显示了一定的α-葡萄糖苷酶抑制活性。
Objective To study the chemical constituents from the leaves of Platycladus orientalis, as well as their antioxidant and α-glucosidase inhibitory activities. Methods The compounds were isolated and purified by silica gel, MCI, polyamide, and prep-HPLC chromatography, their structures were elucidated by spectral analysis. DPPH and ABTS methods were used to study the antioxidant activity, and pNPG method was used to study the α-glucosidase inhibitory activity. Results Eleven compounds(1—11) were isolated from the 80% ethanol extract of the leaves of P. orientalis, and identified as 4-O-(1′,3′-dihydroxypropan-2′-yl)-dihydroconiferyl alcohol 9-O-β-D-glucopyranoside(1), myricetrin(2), 5,8,3′,4′-tetrahydroxy-flavone-7-O-β-D-xylopyranoside(3), isomassonianoside B(4),(-)-isopramine 9′-O-β-D-glucopyranoside(5),(7 R,8 S,7′S,8′R)-4,9,4′,7′-tetrahydroxy-3,3′-dimethoxy-7,9′-epoxylignan 4-O-β-D-glucopyranoside(6), sugiol(7), totarol(8), 5,6-dehydrosugiol methyl ether(9), isopimara-8,15-dien-7-one(10) and ethanol α-L-rhamnopyranoside(11). Conclusion Compound 1 is a new compound, named as platycloside A;In the known compounds, seven compounds(4—7, 9—11) are isolated from this plant for the first time, six compounds(4—6, 9—11) are isolated from the family Thujoideae for the first time, and four compounds(5, 6, 10, 11) are isolated from the family Cupresaceae for the first time. The compounds 2—6 showed a degree of antioxidant activities. The compounds 2 and 3 showed the α-glucosidase inhibitory activities.
作者
吴利苹
俞雅芮
刘梦影
江虹雨
钟思雨
李玲蕊
陆世惠
安梦琪
黄静
WU Li-ping;YU Ya-rui;LIU Meng-ying;JIANG Hong-yu;ZHONG Si-yu;LI Ling-rui;LU Shi-hui;AN Meng-qi;HUANG Jing(West China School of Pharmacy,Sichuan University,Chengdu 610041,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第3期563-570,共8页
Chinese Traditional and Herbal Drugs
作者简介
吴利苹(1995—),女,硕士研究生,从事天然药物化学方面研究。E-mail:1741518396@qq.com;通信作者:黄静(1961—),男,博士,教授,从事天然药物化学方面研究。E-mail:huangj_pharm@scu.edu.cn
