摘要
目的采用生物信息学方法分析细粒棘球绦虫表面抗原EpC1的结构和功能等。方法从NCBI数据库中下载EpC1氨基酸序列,采用ProtParam预测蛋白的理化性质,SignalP-5预测信号肽,Euk-mPLoc 2.0进行亚细胞定位,ProtScale、SOSUI、DNASTAR预测其亲疏水性,SOMPA预测二级结构,NetPhos预测磷酸化位点,MotifScan预测修饰位点,Swissmodel预测三级结构,TMHMM分析跨膜区域;运用ABCpred、IEDB预测B细胞抗原表位,SYFPEITHI预测T细胞抗原表位。结果预测EpC1由174氨基酸序列组成,分子式为C_(884)H_(1403)N_(245)O_(268)S_(9),不稳定指数为46.27,为亲水蛋白,无信号肽和无跨膜区,且定位于细胞膜及细胞质中。二级结构中α螺旋占44.25%,β折叠占14.94%,β转角占5.75%,无规则卷曲占35.06%,EgEpC1具有能与HLA-A*02-01的结合能力,且能被HLA-DRB*0401(DR4Dw4)分子呈递。结论生物信息技术分析EpC1蛋白存在多个B、T细胞表位,含有钙结合结构域,可为该蛋白的基因克隆表达及细粒棘球绦虫感染的防治研究提供理论基础。
Objective To bioinformatically analyze the structure and function of Echinococcus granulosus surface antigen EpC1.Methods The amino acid sequence of EpC1 was downloaded from the NCBI database.The physical and chemical properties of the protein were predicted using ProtParam,its signal peptides were predicted using SignalP-5,its subcellular localization was predicted using Euk-mPLoc 2.0,its hydrophobicity was predicted using ProtScale,SOSUI,and DNASTAR,its secondary structure was predicted using SOMPA,its phosphorylation sites were predicted using NetPhos,its modification sites were predicted using MotifScan,its tertiary structure was predicted using Swissmodel,and its transmembrane regions were predicted using TMHMM.Its B-cell epitopes were predicted using ABCpred and IEDB,and its T-cell epitopes were predicted using SYFPEITHI.Results EpC1 was predicted to consist of a sequence of 174 amino acids,its molecular formula was C_(884)H_(1403)N_(245)O_(268)S_(9),and its instability index was 46.27.It was a hydrophilic protein with no signal peptides and no transmembrane regions,and it was located in the cell membrane and cytoplasm.α-helices accounted for 44.25%of the protein’s secondary structure,β-sheets accounted for 14.94%,β-turns accounted for 5.75%,and random coils accounted for 35.06%.EgEpC1 has the ability to combine with HLA-A*02-01 and can be presented by HLA-DRB*0401(DR4 Dw4)molecules.Conclusion A bioinformatic analysis revealed that the EpC1 protein has multiple B-and T-cell epitopes and that it contains calcium-binding domains.These findings provide a theoretical basis for gene cloning and expression of E.granulosus and prevention and treatment of echinococcosis.
作者
路鹏霏
包永星
田梦潇
赵商岐
武娟
郭刚
齐文静
李军
LU Peng-fei;BAO Yong-xing;TIAN Meng-xiao;ZHAO Shang-qi;WU Juan;GUO Gang;QI Wen-jing;LI Jun(Tumor Center of the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830011,China;State Key Laboratory of Causes and Prevention of High Disease in Central Asia,Research Institute of Clinical Medicine,First Affiliated Hospital of Xinjiang Medical University,Urumqi)
出处
《中国病原生物学杂志》
CSCD
北大核心
2021年第11期1259-1262,1266,共5页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.32072886,81860360)
作者简介
路鹏霏(1991-),男,新疆人,在读博士。研究方向:放射肿瘤学,包虫病学。E-mail:120080903@qq.com;通讯作者:李军,E-mail:1742712944@qq.com
