摘要
目的分析细粒棘球绦虫原头节特异性抗原Eg-00512的生物信息学特征。方法通过NCBI数据库获取Eg-00512蛋白的氨基酸序列;使用ProtParam在线软件分析Eg-00512蛋白的理化性质;采用SOMPA在线软件分析蛋白的二级结构;使用IEDB在线软件预测Eg-00512蛋白的亲水性、柔韧性、表面可及性、β-转角;应用SWISS-MODEL在线服务器分析蛋白氨基酸序列并组合模板和Eg-00512蛋白的三级结构;B细胞表位采用在线软件IEDB及BcePred综合预测,T细胞表位采用在线预测软件SYFPEITHI分析确定。结果Eg-00512蛋白由1254个氨基酸组成,理论pI值6.15;归类于稳定蛋白质,属于亲水性蛋白。Eg-00512蛋白的二级结构中α螺旋占51.28%,延伸链占17.22%,β-转角占4.78%,无规卷曲占26.71%。Eg-00512蛋白B细胞表位数量各为8个;有13个CTL和14个Th细胞优势表位。结论生物信息学方法预测细粒棘球绦虫原头节含有多个优势B、T细胞抗原表位,为包虫病的诊断和疫苗的研究奠定了理论基础。
Objective The bioinformatics characteristics of echinococcus granulosus protoscolex specific antigen EG-00512 were analyzed.Methods The amino acid sequence of Eg-00512 protein from E.granulosus was obtained from the NCBI database.The physical and chemical properties of EG-00512 protein were analyzed by using ProtParam online software.The secondary structure of the protein was analyzed by online software SOMPA.IEDB online software was used to predict the surface accessibility,β-turn,hydrophilic,flexibility.The tertiary structure of Eg-00512 protein was constructed using SWISS-Model online software.The online software IEDB and BcePred were used to predict B cell epitopes.T cell epitopes were analyzed by online prediction software SYFPEITHI.Results Eg-00512 protein was composed of 1254 amino acids with a theoretical pI value of 6.15.Classified as stable proteins,hydrophilic proteins.In the secondary structure of EG-00512 protein,α-helix for 51.28%,extended chain accounted for 17.22%,β-turns for 4.78%,random coils for 26.71%.IEDB and BcePred software were used to predict the B-cell epitopes of EG-00512 protein,and the number of potential epitopes was 8,respectively.SYFPEITHI online software predicted CTL and Th cell epitopes,and there were 13 CTL and 14 Th cell epitopes.Conclusion The B cell and T cell epitopes of E.granulosus EG-00512 protein can be predicted by biological information method,which provides a theoretical basis for the diagnosis and vaccine research of echinococcus disease.
作者
李莎莎
陶佳
吕咏雪
朱亚洲
赵殷奇
赵嘉庆
赵巍
LI Sha-sha;TAO Jia;LV Yong-xue;ZHU Ya-zhou;ZHAO Yin-qi;ZHAO Jia-qing;ZHAO Wei(Department of Basic Medicine,NingXia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,Chnia;General Hospital of Ningxia Medical University;Ningxia Key Laboratory for Prevention and Treatment of Common Infectious Diseases;Ningxia Institute of Medical Science)
出处
《中国病原生物学杂志》
CSCD
北大核心
2022年第1期71-74,113,共5页
Journal of Pathogen Biology
基金
宁夏回族自治区重点研发计划项目(No.2019BCG01001)
宁夏自然科学基金项目(No.2020AAC02039)。
作者简介
通讯作者:赵巍,E-medl:zw-6915@163.com;李莎莎(1984-),女,宁夏人,硕士研究生,主要从事基础分子生物学与寄生虫免疫研究。E-mail:18309679166@163.com。
