摘要
除草剂污染对海洋环境以及水产品质量安全的影响已引起广泛关注。为探究异丙甲草胺污染对黄河三角洲滩涂贝类养殖的影响,本研究以四角蛤蜊(Mactraveneriformis)为实验对象,探讨不同质量浓度异丙甲草胺胁迫对四角蛤蜊鳃和肝胰腺抗氧化酶活性和组织结构的影响,测定胁迫阶段和净水消除阶段不同时间点各组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、过氧化氢酶(catalase,CAT)活性和丙二醛(malondialdehyde,MDA)含量变化,观察其组织的显微结构变化。结果显示,不同浓度的异丙甲草胺胁迫对抗氧化酶活性表现出诱导或抑制作用,0.5μg/L、2.5μg/L异丙甲草胺可对鳃SOD活性、CAT活性造成不可逆的抑制,而10μg/L质量浓度组鳃SOD、CAT活性在净水消除阶段可恢复至对照组水平。3个浓度组的肝胰腺SOD、CAT活性和MDA含量在净水消除阶段可恢复至(或接近)对照组水平。同时异丙甲草胺胁迫造成了鳃丝肿大、纤毛脱落、上皮细胞受损以及消化管破损。结论认为,异丙甲草胺对四角蛤蜊的肝胰腺和鳃均具有明显的剂量效应与时间效应。
The effects of herbicide pollution on the marine environment and aquatic product quality and safety has been of great concern.To explore the effects of metolachlor pollution on shellfish cultivation in the Yellow River Delta,the effects of different mass metolachlor concentrations on gill and hepatopancreas antioxidant enzyme activities and tissue structure of Mactra veneriformis were investigated.Additionally,the tissues at different times during the exposure and elimination phases were determined.Superoxide dismutase(SOD),catalase(CAT)activity,variations in malondialdehyde(MDA)content,and changes to the microstructure of tissues were observed.The results showed that metolachlor induced or inhibited anti-oxidase activity under differing metolachlor concentrations.It was found that 10μg/L of metolachlor significantly increased SOD activity in gill tissue,and different concentrations of metolachlor inhibited CAT activity in the gill and hepatopancreas.Metolachlor at 0.5 and 2.5μg/L caused irreversible oxidative damage to SOD and CAT in the gills;the SOD and CAT activities in the gills of the 10μg/L metolachlor concentration group was restored to the level of the control group during the elimination phase.The SOD and CAT activities in the hepatopancreas of the three concentrations groups were restored to or close to the level of the control group during elimination phase.Compared with SOD,the CAT activity was more susceptible to exogenous stimulation and was inhibited;this may be related to the sensitivity and mechanism of action of the two enzymes.After eliminating drug stress,the MDA content in the gills and hepatopancreas of all concentration groups had returned to or was lower than that of the control group.This suggests that the effects of metolachlor stress on lipid peroxidation in the gills and hepatopancreas of Mactra veneriformis may be reversible.Metolachlor stress induced gill filament enlargement,ciliary shedding,epithelial cell damage,and digestive canal damage;the dose-time effect of two types of tissue damage was evident.However,the effect of drug stress on antioxidant enzymes in tissues did not exhibit a significant dose-effect;this does not correspond to tissue structure damage.It is speculated that there was insufficient damage caused by drug stress to breakdown the antioxidant enzyme system.Different concentrations of drug stress activated various antioxidant enzymes to maintain the physiological balance of the body;this aspect requires further investigation.The results of this study offers a reference for the risk assessment of marine ecosystems and and achieving a controllable level of quality in shellfish aquaculture in tidal areas.
作者
彭中校
黄会
王玮云
任利华
刘鸽
赵军强
宫向红
张秀珍
PENG Zhongxiao;HUANG Hui;WANG Weiyun;REN Lihua;LIU Ge;ZHAO Junqiang;GONG Xianghong;ZHANG Xiuzhen(Shandong Marine Resource and Environment Research Institute,Yantai 264006,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2022年第4期574-584,共11页
Journal of Fishery Sciences of China
基金
山东省重点研发计划项目(2018GHY115041)
关键词
异丙甲草胺
四角蛤蜊
抗氧化酶活性
组织结构
metolachlor
Mactra veneriformis
antioxidant enzyme activity
tissue structure
作者简介
彭中校(1995-),男,硕士研究生,研究方向为水产品质量与安全.E-mail:1578800380@qq.com;通信作者:张秀珍,研究员,研究方向为水产品质量安全与标准化.E-mail:zxz0535501@126.com
