摘要
目的:探究褐变黑枸杞对紫外照射损伤果蝇寿命及抗氧化能力的影响。方法:收集2d龄羽化未交配的雌性果蝇1200只,平均分为6组:正常组、模型组和不同剂量褐变黑枸杞组(3mg/mL、12mg/mL)和红枸杞组(3mg/mL、12mg/mL),除正常组外其余各组每天在紫外强度为20w的紫外灯下照射15min,统计果蝇平均寿命、最高寿命及半数致死时间。同时测定褐变黑枸杞对紫外照射20d果蝇超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性以及丙二醛(MDA)含量的影响。结果表明:两种枸杞均能延长果蝇寿命,但相较于红枸杞,12mg/mL褐变黑枸杞能显著提高紫外照射果蝇的平均寿命、半数死亡时间和最高寿命,且具有剂量依赖性,其中SOD和CAT活性显著升高,MDA含量降低。结论:褐变黑枸杞具有抗氧化损伤、延缓衰老的作用,可用于开发枸杞新产品。
Objective:To explore the effect of brown black wolfberry on the life-span of ultraviolet damaged Drosophila Melanogasterand its anti-oxidation ability.Methods:Colleting 1200 female and male Drosophila at 2 d of born and then divided randomLy into 6 groups:the control group(0 mg/mL,UV radiation 0 min),the model group(0 mg/mL,UV radiation 15 min),brown black wolfberry groups(3 mg/mL,12 mg/mL,UV radiation 15 min),and red wolfberry groups(3 mg/mL,12 mg/mL,UV radiation 15 min).In life-span study,dead flies were counted every 3 days until all flies died;the mean life span,maximum span and 50%survival time were calculated.In anti-oxidation study,SOD activity,CAT activity and MDA content of flies were measured after 20 days of wolferry treated.Results:The results showed that brown black wolfberry could improve the life span of UV-damaged Drosophila Melanogaster.The activities of SOD and CAT was increased with the decreasing of MDA content at the same time.Conclusion:It was concluded that brown black wolfberry has the effect of anti-oxidation and anti-aging.
作者
张静静
刘暄
赵琦
孙立杨
潘佳慧
肖瑶
张泽生
ZHANG Jing-jing;LIU Xuan;ZHAO Qi;SUN Li-yang;PAN Jia-hui;XIAO Yao;ZHANG Ze-sheng(Institute of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457;State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457;Tianjin Food Safety&Low Carbon Manufacturing Collaborative Innovation Center,Tianjin 300457)
出处
《中国食品添加剂》
CAS
2020年第1期53-58,共6页
China Food Additives
关键词
褐变黑枸杞
红枸杞
紫外损伤
果蝇
抗氧化
brown black wolfberry
red wolfberry
ultraviolet-damaged
Drosophila Melanogaster
anti-oxidant
作者简介
张静静(1994-),女(汉),硕士研究生,研究方向:天然产物活性成分;通讯作者:张泽生(1956-),男(汉),教授,博士,研究方向:天然产物活性成分。
