摘要
植物病毒病对世界农业生产构成严重威胁,保护粮食作物不受病毒侵害是农业的一个重大挑战。由分子生物技术主导的抗病毒工程已成为预防和控制植物病毒的有效策略。近期,CRISPR/Cas基因编辑系统介导的靶向DNA或RNA的进展,使其成为适用于植物保护领域极具吸引力的工具,正在被广泛用于生产抗病毒的栽培作物品种。本综述回顾CRISPR/Cas系统的发展,总结它们通过针对病毒序列或寄主易感基因控制不同植物病毒的应用;列出一些可用于抗病毒育种的潜在隐性抗性基因,并强调基于隐性抗性基因的抗病毒育种对产生无发育缺陷的转基因植物的重要性;描述CRISPR/Cas技术在田间检测和诊断植物病毒方面的应用。这一新兴分子技术的研究成果可以为扩展农业和粮食安全的发展作出重大贡献。
Plant virus diseases pose a serious threat to agricultural production worldwide,and protecting food crops from viruses is a major challenge in agriculture.Plant antiviral engineering using molecular biotechnology has become an effective strategy for the prevention and control of plant viruses.Recent advances in the CRISPR/Cas gene editing system-mediated targeting of DNA or RNA have made it a very attractive tool applicable to the field of plant protection and it is being widely used to produce virus-resistant cultivars of crops.This paper reviews the development of CRISPR/Cas systems and summarizes their application in the control of various plant viruses by targeting viral sequences or host susceptibility genes.In addition,some potential recessive resistance genes that can be used for virus resistance breeding are listed,and the importance of recessive resistance gene-based virus resistance breeding for generating developmentally defect-free transgenic plants is emphasized.Finally,the application of CRISPR/Cas technology for the detection and diagnosis of plant viruses in the field is described.The results of this emerging molecular technology can make a significant contribution to the development of agriculture and food security.
作者
沈川
李夏
吴云锋
SHEN Chuan;LI Xia;WU Yunfeng(Shaannan Eco-economy Research Center,Ankang University,Ankang 725000,China;Ankang Academy of Agricultural Sciences,Ankang 725000,China;Northwest A&F University,Yangling 712100,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第3期343-357,共15页
Acta Phytopathologica Sinica
基金
安康学院高层次人才启动专项(2021AYQDZR13)
安康市科技计划项目(AK2021-NY-15)
作者简介
通信作者:沈川,讲师,主要从事植物病毒致病机制研究,Tel:18792805578,E-mail:chuan_shen@aku.edu.cn。
