摘要
目的:研究生黄精、九制黄精及九制黄精复方对抑郁雌性大鼠行为学及性激素水平的影响。方法:SD雌性大鼠随机分为正常对照组、模型对照组、盐酸帕罗西汀1.8 mg/kg组、生黄精10 g/kg组、九制黄精10 g/kg组、九制黄精复方10 g/kg组。采用慢性不可预知性温和刺激(chronic unpredictable mild stress,CUMS)制备抑郁模型,造模6 w后灌胃给药,持续给药21 d,采用蔗糖偏好试验和新奇摄食抑制试验评价抑郁样行为;通过观察阴道脱落细胞检测动情周期;ELISA法检测血清中促性腺激素释放激素(ovarianotropin-releasing hormone,GnRH)、卵泡刺激素(Follicle-stimulating hormone,FSH)、促黄体生成素(Luteinizing hormone,LH)、雌二醇(Estradiol,E2)、孕酮(progesterone,PROG)的浓度;计算卵巢和子宫的脏器指数;HE染色观察卵巢组织病理形态学变化。结果:与正常对照组比较,模型对照组大鼠的体质量和蔗糖偏好率显著降低,在新环境中摄食延迟时间显著延长(P<0.01);动情周期发生紊乱,血清中GnRH、FSH和LH的浓度显著升高,而E2和PROG的浓度显著降低,卵巢指数显著下降(P<0.01),卵巢内生长卵泡减少且闭锁卵泡增多;与模型对照组比较,各给药组大鼠体质量显著增加(P<0.01),蔗糖偏好率明显升高(P<0.05),九制黄精组、九制黄精复方在新环境中摄食延迟时间明显降低(P<0.05);九制黄精组及九制黄精复方组大鼠血清中FSH、LH的浓度明显降低(P<0.05),E2和PROG的浓度显著升高(P<0.01);各给药组动情周期的紊乱得到改善;卵巢组织病理学损伤减轻。结论:九制黄精及九制黄精复方可以改善CUMS抑郁大鼠的抑郁样行为和性激素分泌紊乱,且效果比生黄精更明显。
Objective:To investigate the effects of raw Huangjing(黄精),Jiuzhi Huangjing(九制黄精),and Jiuzhi Huangjing compound on the behaviors and serum sex hormones of female rats with depression.Methods:Female SD rats were randomly divided into a normal control group,a model control group,a positive control group(paroxetine hydrochloride,1.8 mg/kg),a raw Huangjing group(10 mg/kg),a Jiuzhi Huangjing group(10 mg/kg),and a Jiuzhi Huangjing compound group(10 mg/kg).Chronic unpredictable mild stress(CUMS)was used to induce a depression model.Six weeks after modeling,rats were administered orally with corresponding drugs for 21 days.The sucrose preference test and novelty suppressed feeding test were used to evaluate the depression-like behaviors of rats.The exfoliated cells from vagina were observed to detect the estrous cycle.The serum levels of gonadotrophin-releasing hormone(GnRH),follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),and progesterone(PROG)were determined by ELISA.The organ indexes of the ovary and uterus were calculated,and the histopathological change in the ovary was observed by HE staining.Results:Compared with the normal control group,the model control group showed decreased body weight and sucrose preference,prolonged feeding time in the novelty suppressed feeding test(P<0.01),disordered estrous cycle,increased serum levels of GnRH,FSH,and LH,reduced levels of E2 and PROG,declining ovarian index(P<0.01),dwindled developing follicles,and increased atretic follicles.Compared with the model control group,the groups with drug treatment showed increased body weight(P<0.01)and elevated sucrose preference(P<0.05).The Jiuzhi Huangjing group and the Jiuzhi Huangjing compound group displayed shortened feeding time in the novelty suppressed feeding test(P<0.05),decreased serum levels of FSH and LH(P<0.05),and increased levels of E2 and PROG(P<0.01).The disorder of the estrous cycle was ameliorated and the histopathological damage to ovarian tissues was relieved in all groups with drug treatment.Conclusion:Jiuzhi Huangjing and Jiuzhi Huangjing compound can improve the depression-like behaviors and the disorder of sex hormone secretion in CUMS-induced depression rats,and the effects are superior to that of raw huangjing.
作者
李昕燃
何先元
章蓝月
Li Xinran;He Xianyuan;Zhang Lanyue(Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases,Chongqing 400016;College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 401331)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2022年第2期131-136,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
重庆市科技局社会民生类一般项目(编号:cstc2018jscx-msybX0288)
重庆科卫联合中医药技术创新与应用发展重点项目(编号:2021ZY013872)
关键词
黄精
抑郁
大鼠
行为
性激素
Huangjing(黄精)
depression
rats
behavior
sex hormones
作者简介
通信作者:何先元,教授,主要从事中药资源开发与应用,中药材质量控制研究,E-mail:hxy9418@126.com;李昕燃,生药学专业硕士研究生,E-mail:964164351@qq.com。
