摘要
为探讨嘉宝果(Myrciaria cauliflora)叶片多酚的分离纯化方法,对4种树脂(NKA-2、NKA-9、HPD-826和HPD-400A)进行了筛选,并分析了其多酚的抗氧化、体外降糖活性和组成成分。结果表明,NKA-9树脂适于嘉宝果叶片多酚纯化,最佳工艺条件为:上样液质量浓度2.00 mg/mL、洗脱液乙醇体积分数70%、上样流速1.0 mL/min、上样量204 mL、洗脱流速0.9mL/min、洗脱量70 mL。嘉宝果叶多酚纯度可达69.86%。嘉宝果叶片纯化后的多酚抗氧化及α-葡萄糖苷酶抑制活性高于纯化前,但α-淀粉酶抑制活性低于纯化前。HPLC结果表明,嘉宝果叶片中含有杨梅苷、芦丁、金丝桃苷和鞣花酸,其中鞣花酸含量最高[(16.15±0.49)mg/g]。因此,NKA-9树脂适合分离纯化嘉宝果叶片多酚,纯化后的多酚抗氧化及α-葡萄糖苷酶抑制活性增强。
To study the separation and purification of polyphenols from Myrciaria caulifloraleaves,four resins,such as NKA-2,NKA-9,HPD-826 and HPD-400A,were screened,andthe antioxidant,in vitrohypoglycemic activities and components of polyphenols were analyzed.The results showed that NKA-9 resin was suitable for the purification of polyphenols from the leaves.The optimum conditions were as follows:sample concentration of 2.00 mg/mL,flow rate at1.0 mg/mL and sample loading of204 mL,70 mL 70%(V/V)ethanol as elutionwith velocity at0.9 mL/min.Under the conditions,the purity of polyphenols from the leaves could reach 69.86%.The antioxidant andα-glucosidase inhibitory activities of purified polyphenols from leaves of M.cauliflorawere stronger than those of crude polyphenols,but the inhibitory activities onα-amylase were lower.According to HPLC,themonophenols in leaves of M.caulifloracontainedmyricetin,rutin,hyperoside and ellagic acid,and ellagic acid content was highest with(16.15±0.49)mg/g.Therefore,NKA-9 resin was suitable for separationand purification of polyphenols from leaves ofM.cauliflora,the antioxidant andα-glucosidase inhibitory activities of purified polyphenols increased.
作者
林宝妹
邱珊莲
张帅
吴妙鸿
张少平
洪佳敏
LIN Baomei;QIU Shanlian;ZHANG Shuai;WU Miaohong;ZHANG Shaoping;HONG Jiamin(Fujian Academy of Agricultural Sciences,Institute of Subtropical Agriculture,Zhangzhou 363005,Fujian,China)
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2021年第5期563-572,共10页
Journal of Tropical and Subtropical Botany
基金
福建省科技计划公益类专项(2019R1030-4)
福建省自然科学基金项目(2020J011369)
福建省农业科学院青年创新团队项目(STIT2017-3-4)资助。
关键词
多酚
树脂
嘉宝果
抗氧化
降血糖
化学成分
Polyphenol
Resin
Jaboticaba
Antioxidation
Hypoglycemic
Chemicalcomposition
作者简介
林宝妹(1988-),女,助理研究员,研究方向为天然产物化学。E-mail:yogobm@qq.com;通信作者:邱珊莲.E-mail:slqiu79@163.com。
