摘要
为了进一步全面地阐明荆芥药材乙醇提取物中主要发挥抗肺癌作用的化学组成,本文基于微流控芯片技术开展不同产地荆芥药材乙醇提取物的体外抗肺肿瘤药效学研究,并通过HPLC方法建立9种不同产地荆芥药材乙醇提取物的指纹图谱,将药效指标与指纹图谱的相关信息通过灰色关联度软件进行谱效关系分析,采用HPLC-Q-TOF/MS的技术手段对其进行快速分析与鉴定。结果显示,关联系数较大的色谱峰中第19、6、16、11、18号峰所代表的化合物为香叶木素、木犀草苷、木犀草素、橙皮苷、芹菜素;第10、12、20号峰可能为柚皮素-7-O-葡萄糖醛酸苷或槲皮苷、迷迭香酸甲酯或异鼠李素-3-木糖苷、5,7-二羟基-6,4-二甲氧基黄酮。该研究进一步明确了荆芥中发挥抗肺肿瘤作用的主要化学组成,为荆芥药材的质量控制及中药复杂成分的药效物质基础研究提供方法学探讨和实验依据。
This study was designed to elucidate the chemical composition and anti-cancer effects of Schizonepeta tenuifolia＇s ethanol extracts. Microfluidic technology was used in the study of Schizonepeta tenuifolia from 9 different geographic regions. The ethanol extracts were examined with HPLC to establish their Fingerprints in order to analyze the relationship between the spectrum and efficacy index through Grey Correlation software, and a rapid HPLC-Q-TOF/MS method was established. The result shows that chroma- tographic peaks of the 19, 6, 11, 16, 18th are the representative diosmetin, luteoloside, hesperidin, luteolin, and apigenin. The 10, 12, 20th peaks may be naringenin-7- O-glucuronide or quercitrin, rosmarinate or acetyl- corynoline, and 5,7-dihydroxy-6,4-dimethoxy flavone. The major chemical composition of Schizonepeta tenui- folia was found to have the anti-lung-tumor effects. A new method was established for the quality control of traditional Chinese medicine.
出处
《药学学报》
CAS
CSCD
北大核心
2017年第1期126-131,共6页
Acta Pharmaceutica Sinica
基金
"十二五""重大新药创制"科技重大专项资助项目(2013ZX09507005)
辽宁省一般项目资助项目(L2014361
L201605)
关键词
荆芥
微流控芯片
A549细胞
谱效关系
灰色关联度
Schizonepeta tenuifolia
microfluidic chip
A549 cell
spectrum effect relationship
grey correlation degree
作者简介
通讯作者Tel／Fax：86-411-85890185，E-mail：mxswv@126．com
