2021年7月17—22日河南省发生了一次历史罕见的持续性强降水,造成了巨大的经济损失。目前极端降水预报仍是次季节气候预测研究的热点和难点。区域气候模式有着比全球模式更精细的空间分辨率和更为完善的物理过程参数化方案,为进一步提...2021年7月17—22日河南省发生了一次历史罕见的持续性强降水,造成了巨大的经济损失。目前极端降水预报仍是次季节气候预测研究的热点和难点。区域气候模式有着比全球模式更精细的空间分辨率和更为完善的物理过程参数化方案,为进一步提高中国次季节降水预报能力提供了新途径。使用区域气候模式CWRF(regional Climate-Weather Research and Forecasting model)对中国气象局全球气候模式次季节预测系统CMA_CPSv3(China Meteorological Administration Climate Prediction System version 3)的预报结果进行中国区域动力降尺度,分析了CWRF和CMA_CPSv3模式对河南省2021年7月17—22日持续性强降水的预测效果。结果表明,区域模式和全球模式预报的降水空间分布和量级存在明显差异。尽管两个模式都低估了此次强降水过程的降水量,但总体上CWRF模式预报的降水量更大且更好地捕捉到了降水的空间分布。CWRF模式自6月26和29日起报的降水预报明显好于同一起报日CMA_CPSv3模式的预报结果。与CMA_CPSv3预报相比,CWRF显著地改善了东亚低空风场和低空急流的预报。CWRF对低空急流和水汽通量输送方向的改善尤为明显,预报的水汽在山脉的迎风坡辐合,为降水提供了有利的水汽条件。同时CWRF更好地预报了郑州上空的垂直上升运动,这些改善都有利于CWRF模式对降水有更高的预报技巧。展开更多
Objective:Oxaliplatin(OXA)and 5-fluorouracil(5-FU)are 2 commonly used chemotherapeutic agents for colorectal cancer(CRC).MicroRNAs(miRNAs,miRs)play crucial roles in the development of chemoresistance in various cancer...Objective:Oxaliplatin(OXA)and 5-fluorouracil(5-FU)are 2 commonly used chemotherapeutic agents for colorectal cancer(CRC).MicroRNAs(miRNAs,miRs)play crucial roles in the development of chemoresistance in various cancers.However,the role and mechanism of miR-224-5p in regulating CRC chemoresistance remain unclear.This study aims to investigate the function of miR-224-5p in chemoresistant CRC cells and the underlying mechanisms.Methods:CRC datasets GSE28702 and GSE69657 were downloaded from the Gene Expression Omnibus(GEO)database.Differentially expressed miRNAs between drug sensitive and resistant groups(OXA or 5-FU)were analyzed,and miR-224-5p was identified as the target miRNA.Chemoresistant cell lines HCT15-OXR,HCT15-5-FU,SW480-OXR,and SW480-5-FU were established.Transient transfections were performed using miR-224-5p mimics,inhibitors,and their respective negative controls(control mimic,control inhibitor)in these cell lines.Cells were treated with different concentrations of OXA or 5-FU post-transfection,and the half-maximal inhibitory concentration(IC_(50))was determined using the cell counting kit-8(CCK-8)assay.Cell proliferation was assessed by CCK-8 and colony formation assays.The expression levels of miR-224-5p,LC3,and P62 were measured by real-time polymerase chain reaction(real-time PCR)and/or Western blotting.Autophagic flux was assessed using a tandem fluorescent-tagged LC3 reporter assay.TargetScan 8.0,miRTarBase,miRPathDB,and HADb were used to predict B-cell lymphoma-2(Bcl-2)as a potential miR-244-5p target,which was further validated by dual luciferase reporter assays.Results:Chemoresistant CRC cells exhibited down-regulated miR-224-5p expression,whereas up-regulation of miR-224-5p enhanced chemotherapy sensitivity.Exposure to OXA or 5-FU significantly increased autophagic activity in chemoresistant CRC cells,which was reversed by miR-224-5p overexpression.Dual-luciferase assays verified Bcl-2 as a direct target of miR-224-5p.Conclusion:MiR-224-5p regulates chemoresistance in CRC by modulating autophagy through direct targeting of Bcl-2.展开更多
Background GOSSYM is a mechanistic,process-based cotton model that can simulate cotton crop growth and development,yield,and fiber quality.Its fiber quality module was developed based on controlled experiments explici...Background GOSSYM is a mechanistic,process-based cotton model that can simulate cotton crop growth and development,yield,and fiber quality.Its fiber quality module was developed based on controlled experiments explicitly conducted on the Texas Marker^(-1)(TM1)variety,potentially making its functional equations more aligned with this cultivar.To assess the model’s broader applicability,this study analyzed fiber quality data from 40 upland cotton cultivars,including TM1.The measured fiber quality from all cultivars was then compared with the modelsimulated fiber quality.Results Among the 40 upland cultivars,fiber strength varied from 28.4 cN·tex^(-1) to 34.6 cN·tex^(-1),fiber length ranged from 27.1 mm to 33.3 mm,micronaire value ranged from 2.7 to 4.6,and length uniformity index varied from 82.3%to 85.5%.The model simulated fiber quality closely matched the measured values for TM1,with the absolute percentage error(APE)being less than 0.92%for fiber strength,fiber length,and length uniformity index and 4.7%for micronaire.However,significant differences were observed for the other cultivars.The Pearson correlation coefficient(r)between the measured and simulated values was negative for all fiber quality traits,and Wilmotts’s index of agreement(WIA)was below 0.45,indicating a strong model bias toward TM1 without incorporating cultivar-specific parameters.After incorporating cultivar-specific parameters,the model’s performance improved significantly,with an average r-value of 0.84 and WIA of 0.88.Conclusions The adopted methodology and estimated cultivar-specific parameters improved the model’s simulation accuracy.This approach can be applied to newer cotton cultivars,enhancing the GOSSYM model’s utility and its applicability for agricultural management and policy decisions.展开更多
背景与目的云南东部农村地区宣威市、富源县女性居民主要从事农业生产和家务工作,基本不吸烟,但肺癌死亡率却是世界上最高的,而且发病、死亡年龄提前。本研究对宣威、富源非吸烟女性肺癌生存状况及其影响因素进行分析。方法以2006年-201...背景与目的云南东部农村地区宣威市、富源县女性居民主要从事农业生产和家务工作,基本不吸烟,但肺癌死亡率却是世界上最高的,而且发病、死亡年龄提前。本研究对宣威、富源非吸烟女性肺癌生存状况及其影响因素进行分析。方法以2006年-2010年被当地省、市、县9家医院新诊断、并纳入"非吸烟女性肺癌病例对照研究项目"的常住户籍女性肺癌病例为研究对象随访至2016年末。通过Life-table法进行全部病例生存分析,评估人群相对生存率和年龄别标化相对生存率。应用Kaplan-Meier法和Cox比例风险模型分别进行单因素生存分析、分层分析和多因素分析。结果随访的1,250例病例中,死亡1,075例,删失175例,随访中位时间为69个月(95%CI:61.9-76.0)。病例平均年龄(54.8±10.9)岁,I期、II期、III期、IV期和未知分期分别占3.5%、8.7%、20.7%、29.7%和37.4%;手术、非手术治疗和未治疗分别占17. 2%、39.0%和43. 8%,组织学、细胞学诊断占51.6%。中位生存时间13.2个月,5年观察生存率、相对生存率、年龄标化相对生存率分别为8.9%(95%CI:7.0-10.6)、9.4%(95%CI:7.6-11.5)和10.1%(95%CI:3.7-20.5)。I期、II期、III期、IV期、未分期5年生存率分别为41.1%、22.4%、5. 3%、1. 3%、11.2%;手术治疗、非手术治疗、未治疗分别为34.8%和3.2%、4.7%;腺癌、鳞癌分别为17.9%和5.6%。省级医院治疗、X线胸部筛查、非农民职业、城镇居住、65岁以下年龄等因素有利于提高生存率,而市县级医院治疗、农民职业、乡村居住、65岁以上年龄等则生存率较低。分层分析显示,任意原发灶-淋巴结-远处转移(tumornode-met a st a si s,T N M)分期,无论腺癌或鳞癌患者,行手术治疗的生存率明显高于非手术治疗;与未治疗病例相比非手术治疗仅在III期显示差异;腺癌生存率大于鳞癌不仅仅因为早期和手术病例较多,在III期、未分期也显示明显生存优势。不同级别医院治疗疗效有明显差异,省级医院治疗的IV期、鳞癌的生存预后明显优于市、县级医院。Cox分析显示治疗方法、TNM分期、治疗医院级别、X线胸部筛查是独立预后因素,其中TNM分期、手术治疗对肺癌患者生存影响较大,而治疗医院级别、X胸部筛查相对较弱。结论宣威、富源非吸烟女性肺癌生存率较低,主要与其诊断时早期病例和手术、综合治疗较少、而未治疗病例较多有关,其次较差的农村社会经济、健康保障等也是生存预后的不利因素。展开更多
文摘2021年7月17—22日河南省发生了一次历史罕见的持续性强降水,造成了巨大的经济损失。目前极端降水预报仍是次季节气候预测研究的热点和难点。区域气候模式有着比全球模式更精细的空间分辨率和更为完善的物理过程参数化方案,为进一步提高中国次季节降水预报能力提供了新途径。使用区域气候模式CWRF(regional Climate-Weather Research and Forecasting model)对中国气象局全球气候模式次季节预测系统CMA_CPSv3(China Meteorological Administration Climate Prediction System version 3)的预报结果进行中国区域动力降尺度,分析了CWRF和CMA_CPSv3模式对河南省2021年7月17—22日持续性强降水的预测效果。结果表明,区域模式和全球模式预报的降水空间分布和量级存在明显差异。尽管两个模式都低估了此次强降水过程的降水量,但总体上CWRF模式预报的降水量更大且更好地捕捉到了降水的空间分布。CWRF模式自6月26和29日起报的降水预报明显好于同一起报日CMA_CPSv3模式的预报结果。与CMA_CPSv3预报相比,CWRF显著地改善了东亚低空风场和低空急流的预报。CWRF对低空急流和水汽通量输送方向的改善尤为明显,预报的水汽在山脉的迎风坡辐合,为降水提供了有利的水汽条件。同时CWRF更好地预报了郑州上空的垂直上升运动,这些改善都有利于CWRF模式对降水有更高的预报技巧。
基金supported by the National Natural Science Foundation(82072729)Wuhan Municipal Health Commission Medical Research Project(WX19Q30),China。
文摘Objective:Oxaliplatin(OXA)and 5-fluorouracil(5-FU)are 2 commonly used chemotherapeutic agents for colorectal cancer(CRC).MicroRNAs(miRNAs,miRs)play crucial roles in the development of chemoresistance in various cancers.However,the role and mechanism of miR-224-5p in regulating CRC chemoresistance remain unclear.This study aims to investigate the function of miR-224-5p in chemoresistant CRC cells and the underlying mechanisms.Methods:CRC datasets GSE28702 and GSE69657 were downloaded from the Gene Expression Omnibus(GEO)database.Differentially expressed miRNAs between drug sensitive and resistant groups(OXA or 5-FU)were analyzed,and miR-224-5p was identified as the target miRNA.Chemoresistant cell lines HCT15-OXR,HCT15-5-FU,SW480-OXR,and SW480-5-FU were established.Transient transfections were performed using miR-224-5p mimics,inhibitors,and their respective negative controls(control mimic,control inhibitor)in these cell lines.Cells were treated with different concentrations of OXA or 5-FU post-transfection,and the half-maximal inhibitory concentration(IC_(50))was determined using the cell counting kit-8(CCK-8)assay.Cell proliferation was assessed by CCK-8 and colony formation assays.The expression levels of miR-224-5p,LC3,and P62 were measured by real-time polymerase chain reaction(real-time PCR)and/or Western blotting.Autophagic flux was assessed using a tandem fluorescent-tagged LC3 reporter assay.TargetScan 8.0,miRTarBase,miRPathDB,and HADb were used to predict B-cell lymphoma-2(Bcl-2)as a potential miR-244-5p target,which was further validated by dual luciferase reporter assays.Results:Chemoresistant CRC cells exhibited down-regulated miR-224-5p expression,whereas up-regulation of miR-224-5p enhanced chemotherapy sensitivity.Exposure to OXA or 5-FU significantly increased autophagic activity in chemoresistant CRC cells,which was reversed by miR-224-5p overexpression.Dual-luciferase assays verified Bcl-2 as a direct target of miR-224-5p.Conclusion:MiR-224-5p regulates chemoresistance in CRC by modulating autophagy through direct targeting of Bcl-2.
基金supported by United States Department of Agriculture,Agricultural Research Service(No.58-8042-9-072)United States Department of Agriculture-National Institute of Food and Agriculture(No.2019-34263-30552)+1 种基金Management Information System(No.043050)United States Department of Agriculture-Agricultural Research Service-Non-Assistance Cooperative Agreement(No.58-6066-2-030).
文摘Background GOSSYM is a mechanistic,process-based cotton model that can simulate cotton crop growth and development,yield,and fiber quality.Its fiber quality module was developed based on controlled experiments explicitly conducted on the Texas Marker^(-1)(TM1)variety,potentially making its functional equations more aligned with this cultivar.To assess the model’s broader applicability,this study analyzed fiber quality data from 40 upland cotton cultivars,including TM1.The measured fiber quality from all cultivars was then compared with the modelsimulated fiber quality.Results Among the 40 upland cultivars,fiber strength varied from 28.4 cN·tex^(-1) to 34.6 cN·tex^(-1),fiber length ranged from 27.1 mm to 33.3 mm,micronaire value ranged from 2.7 to 4.6,and length uniformity index varied from 82.3%to 85.5%.The model simulated fiber quality closely matched the measured values for TM1,with the absolute percentage error(APE)being less than 0.92%for fiber strength,fiber length,and length uniformity index and 4.7%for micronaire.However,significant differences were observed for the other cultivars.The Pearson correlation coefficient(r)between the measured and simulated values was negative for all fiber quality traits,and Wilmotts’s index of agreement(WIA)was below 0.45,indicating a strong model bias toward TM1 without incorporating cultivar-specific parameters.After incorporating cultivar-specific parameters,the model’s performance improved significantly,with an average r-value of 0.84 and WIA of 0.88.Conclusions The adopted methodology and estimated cultivar-specific parameters improved the model’s simulation accuracy.This approach can be applied to newer cotton cultivars,enhancing the GOSSYM model’s utility and its applicability for agricultural management and policy decisions.
文摘背景与目的云南东部农村地区宣威市、富源县女性居民主要从事农业生产和家务工作,基本不吸烟,但肺癌死亡率却是世界上最高的,而且发病、死亡年龄提前。本研究对宣威、富源非吸烟女性肺癌生存状况及其影响因素进行分析。方法以2006年-2010年被当地省、市、县9家医院新诊断、并纳入"非吸烟女性肺癌病例对照研究项目"的常住户籍女性肺癌病例为研究对象随访至2016年末。通过Life-table法进行全部病例生存分析,评估人群相对生存率和年龄别标化相对生存率。应用Kaplan-Meier法和Cox比例风险模型分别进行单因素生存分析、分层分析和多因素分析。结果随访的1,250例病例中,死亡1,075例,删失175例,随访中位时间为69个月(95%CI:61.9-76.0)。病例平均年龄(54.8±10.9)岁,I期、II期、III期、IV期和未知分期分别占3.5%、8.7%、20.7%、29.7%和37.4%;手术、非手术治疗和未治疗分别占17. 2%、39.0%和43. 8%,组织学、细胞学诊断占51.6%。中位生存时间13.2个月,5年观察生存率、相对生存率、年龄标化相对生存率分别为8.9%(95%CI:7.0-10.6)、9.4%(95%CI:7.6-11.5)和10.1%(95%CI:3.7-20.5)。I期、II期、III期、IV期、未分期5年生存率分别为41.1%、22.4%、5. 3%、1. 3%、11.2%;手术治疗、非手术治疗、未治疗分别为34.8%和3.2%、4.7%;腺癌、鳞癌分别为17.9%和5.6%。省级医院治疗、X线胸部筛查、非农民职业、城镇居住、65岁以下年龄等因素有利于提高生存率,而市县级医院治疗、农民职业、乡村居住、65岁以上年龄等则生存率较低。分层分析显示,任意原发灶-淋巴结-远处转移(tumornode-met a st a si s,T N M)分期,无论腺癌或鳞癌患者,行手术治疗的生存率明显高于非手术治疗;与未治疗病例相比非手术治疗仅在III期显示差异;腺癌生存率大于鳞癌不仅仅因为早期和手术病例较多,在III期、未分期也显示明显生存优势。不同级别医院治疗疗效有明显差异,省级医院治疗的IV期、鳞癌的生存预后明显优于市、县级医院。Cox分析显示治疗方法、TNM分期、治疗医院级别、X线胸部筛查是独立预后因素,其中TNM分期、手术治疗对肺癌患者生存影响较大,而治疗医院级别、X胸部筛查相对较弱。结论宣威、富源非吸烟女性肺癌生存率较低,主要与其诊断时早期病例和手术、综合治疗较少、而未治疗病例较多有关,其次较差的农村社会经济、健康保障等也是生存预后的不利因素。
基金Key Project Process Mechanism and Prediction of Geological Hazards (2001CB711005-1-3) and State Key Basic Research Project Mechanism and Prediction of Continental Earthquakes (G1998040702). sponsored by the Ministry of Science and Techno
