The microstructure,mechanical properties and corrosion resistance of Zr-30%Ta and Zr-25%Ta-5%Ti alloy prepared by spark plasma sintering(SPS)technology were investigated.The experimental results showed that the Zr-Ta-...The microstructure,mechanical properties and corrosion resistance of Zr-30%Ta and Zr-25%Ta-5%Ti alloy prepared by spark plasma sintering(SPS)technology were investigated.The experimental results showed that the Zr-Ta-Ti alloys made by the SPS processing have a low level of porosity with the relative density of 96%−98%.The analyses of XRD and TEM revealed that the Zr-30Ta alloy consists ofα+βphase,and the Zr-25Ta-5Ti alloy belongs to the nearβtype alloy containing a small amount ofαandωphases.With the addition of Ti,the elastic modulus of the alloys was decreased from(99.5±7.2)GPa for Zr-30Ta alloy to(73.6±6.3)GPa for Zr-25Ta-5Ti alloy.Furthermore,it is shown that,in comparison to CP-Ti and Ti-6Al-4V alloy,the Zr-Ta-Ti alloy produced in this work offers an improved corrosion resistance due to the more stable ZrO2 and Ta2O5 generated in the passivation film on the surface of the alloys.This study demonstrates that Zr-Ta-Ti alloys are a promising candidate of novel metallic biomaterials.展开更多
Osteoarthritis(OA)is an inflammatory disease involving the joints that is prevalent in the global aging population.The purpose of this study is to determine whether irisin can attenuate osteoarthritis(OA)progression i...Osteoarthritis(OA)is an inflammatory disease involving the joints that is prevalent in the global aging population.The purpose of this study is to determine whether irisin can attenuate osteoarthritis(OA)progression in anterior cruciate ligament transection(ACLT)mice models and the mechanism of irisin therapy effect on OA by increase the resistance of apoptosis in MLO-Y4 cells induced by mechanical stretch in vitro.Methods For in vivo study,3-month-old male C57BL/6 J mice were randomized to three groups,sham-operated,anterior cruciate ligament transection(ACLT)-operated treated with vehicle,and ACLT-operated treated with irisin by intraperitoneal injection once a week.Cartilage erosion was observed by HE staining.Osteoarthritis Research Society International(OARSI)scores were evaluated according to the safranin O stai-ning.The microstructure of tibia cortical bone,trabecular bone,and subchondral bone was analyzed by micro-CT and the bone histomorphometry has been administrated including mineral apposition rate(MAR).Edu staining and cck-8 were used for the detection of the proliferation of MLO-Y4 cells.For mechanical stress,cells were seeded on the collagen-I coated chamber subjected with a peak biaxial stretch of 20%at 1 Hz for 16 hours to induce apoptosis.Flow cytometry was used for the detection of apoptosis and cell cycle.TUNNEL was used for staining the apoptotic cells and rt-PCR was applied for quantifying the expression of mRNA such as Bax,Bcl-2,SOST,c-myc,Opg.Western blot was utilized to confirm the mechanism of how irisin decrease the osteocyte apoptosis.Results In vivo,irisin can attenuate articular cartilage degeneration.Irisin maintains the proportion of hyaline cartilage and calcified cartilage and keep fewer cartilage erosions in ACLT-operated mice.For immunohistochemical(IHC)staining,irisin reduced the expression of caspase3,Bax and matrix metalloproteinase-13 in both cartilage and subchondral bone.Irisin-treated ACLT group shows higher Trabecular number(Tb.N)and bone volume fraction(BV/TV)compared to the vehicle-treated ACLT group.In vitro, irisin significantly increased the proliferation of MLO-Y4 cells detected by Edu and Ki67 staining,and irisin can protect the cells from both mechanical stretchinduced apoptosis detected by FITC-PI flow cytometry and maintain the cell activity by regulating the expression of Bax,Bcl-2,and c-myc.Transcriptome sequencing shows that irisin significantly activates the MAPK signaling pathway and we confirm the result by western blot:irisin effectively activates the Erk signaling pathway through phosphorylation and has a certain activation effect on p38 signaling pathway,no activation was observed for FAK signaling pathway.Conclusions Irisin can attenuate the progression of OA by decrease the apoptosis of osteocyte,which can improve the microarchitecture of subchondral bone.Erk pathway activation plays an important role in reducing the apoptosis of osteocyte.展开更多
Objective To investigate the effectiveness of the micro-dynamic pedicle screw for the prevention of loosening between the screw and bone surface in lumbar spinal fusion.For this evaluation,the peek pull-out force(PPF)...Objective To investigate the effectiveness of the micro-dynamic pedicle screw for the prevention of loosening between the screw and bone surface in lumbar spinal fusion.For this evaluation,the peek pull-out force(PPF)after fatigue resistance testing at different cycles and semidiameter of screw insertion area of micro-CT image was compared between the micro-dynamic and traditional pedicle screws.Methods Forty lumbar vertebrae received a traditional pedicle screw on one side and a micro-dynamic pedicle screw on the other side as follows:15 vertebrae underwent instant pull-out testing(group A,traditional vs.group B,micro-dynamic);15 vertebrae underwent pull-out test after 5000-cyclic fatigue loading(group C,traditional vs.group D,micro-dynamic);and 10 vertebrae underwent pull-out test after 10000-cyclic fatigue loading(group E,traditional vs.group F,micro-dynamic).All vertebrae that after fatigue loading were scanned by using micro-ct to check the semidiameter of screw insertion area.The PPF,normalized PPF(PPFn),bone mineral density(BMD)and diameter of screw insertion area were compared between all groups.Results The BMD showed a weak significant correlation with PPF(r=0.252,P=0.024).The PPF in groups A and B(P=0.485),and groups C and D(P=0.184)did not show significant difference.However,the PPF in group F was significantly greater than that measured in group E(P=0.005).The PPFn of groups A,C,and E significantly decreased as the number of cycles increased(P=0.015).Meanwhile,the PPFn of groups B,D,and F remained consistent regardless of the number of cycles(P=0.367). The semidiameter of traditional pedicle screw insertion area was significantly larger than that of micro-dynamic pedicle screw insertion area(P<0.001).Conclusions This study compared the performance of a micro-dynamic pedicle screw versus that of a traditional pedicle screw using pull-out testing and micro-CT.The results showed that the micro-dynamic pedicle screw provides similar resistance in lowcycle fatigue testing and favorable resistance in high-cycle fatigue testing versus the traditional pedicle screw.The micro-dynamic pedicle screw provides stronger fixation stability versus the traditional pedicle screw,especially in high-cyclic fatigue loading condition versus the traditional pedicle screws.展开更多
Objective:While age has been recognized as a noteworthy factor in preoperative graft selection,the correlation between age and hamstring autograft diameter has been the subject of continued debate within the scientifi...Objective:While age has been recognized as a noteworthy factor in preoperative graft selection,the correlation between age and hamstring autograft diameter has been the subject of continued debate within the scientific community.This study aimed to explore the correlation between the diameter of autologous tendon grafts and age in anterior cruciate ligament reconstruction(ACLR).Methods:A retrospective review of 388 patients who underwent arthroscopic ACLR with hamstring autografts was performed.Patients were grouped by age to analyze differences in hamstring autograft dia-meter and tendon cross-sectional area(CSA).We explored the correlations between age and graft diameter and between age and the CSA of the popliteal tendon while controlling for the influence of other pertinent variables.Results:Compared with female patients,male patients presented significantly greater autograft diameters and hamstring tendon CSAs(P<0.05).Notably,graft diameter and hamstring tendon CSA varied significantly across different age groups(P<0.05);patients aged>32 years were substantially more likely to have a graft diameter exceeding 8 mm and a CSA surpassing 18.5 mm^(2) than their≤32-year-old counterparts were(P<0.05).Conclusion:This study revealed that graft diameter varies across different age groups,with age independently influencing graft diameter.展开更多
The major problem with lithium-sulfur(Li-S)batteries is their poor cycling stability because of slow redox kinetics in the cathode and the growth of lithium dendrites on the anode.We report the production of 2D porous...The major problem with lithium-sulfur(Li-S)batteries is their poor cycling stability because of slow redox kinetics in the cathode and the growth of lithium dendrites on the anode.We report the production of 2D porous carbon nanosheets doped with both Fe and Ni(Fe/Ni-N-PCNSs)by an easy and template-free approach that solve this problem.Because of their ultrathin porous 2D structure and uniform distribution of Fe and Ni dopants,they capture polysulfides,speed up the sulfur redox reaction,and improve the material’s lithiophilicity,greatly suppressing the shuttling of polysulfides and dendrite growth on the lithium anode.As a result,it has an exceptional performance as a stable host for elemental sulfur and metallic lithium,producing a record long life of 1000 cycles with a very small capacity decay of 0.00025%per cycle in a Li-S battery and an excellent cycling stability of over 850 h with a small overpotential of>72 mV in a lithium metal battery.This work suggests the use of multifunctional-based 2D porous carbon nanosheets as a stable host for both elemental sulfur and metallic lithium to improve the Li-S battery per-formance.展开更多
基金Project(51404302)supported by the National Natural Science Foundation of ChinaProject(QJ2018003A)supported by the Youth Scientific Research Foundation of the Central South University of Forestry and Technology,China。
文摘The microstructure,mechanical properties and corrosion resistance of Zr-30%Ta and Zr-25%Ta-5%Ti alloy prepared by spark plasma sintering(SPS)technology were investigated.The experimental results showed that the Zr-Ta-Ti alloys made by the SPS processing have a low level of porosity with the relative density of 96%−98%.The analyses of XRD and TEM revealed that the Zr-30Ta alloy consists ofα+βphase,and the Zr-25Ta-5Ti alloy belongs to the nearβtype alloy containing a small amount ofαandωphases.With the addition of Ti,the elastic modulus of the alloys was decreased from(99.5±7.2)GPa for Zr-30Ta alloy to(73.6±6.3)GPa for Zr-25Ta-5Ti alloy.Furthermore,it is shown that,in comparison to CP-Ti and Ti-6Al-4V alloy,the Zr-Ta-Ti alloy produced in this work offers an improved corrosion resistance due to the more stable ZrO2 and Ta2O5 generated in the passivation film on the surface of the alloys.This study demonstrates that Zr-Ta-Ti alloys are a promising candidate of novel metallic biomaterials.
基金supported by the National Natural Science Foundation of China ( 31670957)
文摘Osteoarthritis(OA)is an inflammatory disease involving the joints that is prevalent in the global aging population.The purpose of this study is to determine whether irisin can attenuate osteoarthritis(OA)progression in anterior cruciate ligament transection(ACLT)mice models and the mechanism of irisin therapy effect on OA by increase the resistance of apoptosis in MLO-Y4 cells induced by mechanical stretch in vitro.Methods For in vivo study,3-month-old male C57BL/6 J mice were randomized to three groups,sham-operated,anterior cruciate ligament transection(ACLT)-operated treated with vehicle,and ACLT-operated treated with irisin by intraperitoneal injection once a week.Cartilage erosion was observed by HE staining.Osteoarthritis Research Society International(OARSI)scores were evaluated according to the safranin O stai-ning.The microstructure of tibia cortical bone,trabecular bone,and subchondral bone was analyzed by micro-CT and the bone histomorphometry has been administrated including mineral apposition rate(MAR).Edu staining and cck-8 were used for the detection of the proliferation of MLO-Y4 cells.For mechanical stress,cells were seeded on the collagen-I coated chamber subjected with a peak biaxial stretch of 20%at 1 Hz for 16 hours to induce apoptosis.Flow cytometry was used for the detection of apoptosis and cell cycle.TUNNEL was used for staining the apoptotic cells and rt-PCR was applied for quantifying the expression of mRNA such as Bax,Bcl-2,SOST,c-myc,Opg.Western blot was utilized to confirm the mechanism of how irisin decrease the osteocyte apoptosis.Results In vivo,irisin can attenuate articular cartilage degeneration.Irisin maintains the proportion of hyaline cartilage and calcified cartilage and keep fewer cartilage erosions in ACLT-operated mice.For immunohistochemical(IHC)staining,irisin reduced the expression of caspase3,Bax and matrix metalloproteinase-13 in both cartilage and subchondral bone.Irisin-treated ACLT group shows higher Trabecular number(Tb.N)and bone volume fraction(BV/TV)compared to the vehicle-treated ACLT group.In vitro, irisin significantly increased the proliferation of MLO-Y4 cells detected by Edu and Ki67 staining,and irisin can protect the cells from both mechanical stretchinduced apoptosis detected by FITC-PI flow cytometry and maintain the cell activity by regulating the expression of Bax,Bcl-2,and c-myc.Transcriptome sequencing shows that irisin significantly activates the MAPK signaling pathway and we confirm the result by western blot:irisin effectively activates the Erk signaling pathway through phosphorylation and has a certain activation effect on p38 signaling pathway,no activation was observed for FAK signaling pathway.Conclusions Irisin can attenuate the progression of OA by decrease the apoptosis of osteocyte,which can improve the microarchitecture of subchondral bone.Erk pathway activation plays an important role in reducing the apoptosis of osteocyte.
基金supported by the National Natural Science Foundation of China ( 31670957)
文摘Objective To investigate the effectiveness of the micro-dynamic pedicle screw for the prevention of loosening between the screw and bone surface in lumbar spinal fusion.For this evaluation,the peek pull-out force(PPF)after fatigue resistance testing at different cycles and semidiameter of screw insertion area of micro-CT image was compared between the micro-dynamic and traditional pedicle screws.Methods Forty lumbar vertebrae received a traditional pedicle screw on one side and a micro-dynamic pedicle screw on the other side as follows:15 vertebrae underwent instant pull-out testing(group A,traditional vs.group B,micro-dynamic);15 vertebrae underwent pull-out test after 5000-cyclic fatigue loading(group C,traditional vs.group D,micro-dynamic);and 10 vertebrae underwent pull-out test after 10000-cyclic fatigue loading(group E,traditional vs.group F,micro-dynamic).All vertebrae that after fatigue loading were scanned by using micro-ct to check the semidiameter of screw insertion area.The PPF,normalized PPF(PPFn),bone mineral density(BMD)and diameter of screw insertion area were compared between all groups.Results The BMD showed a weak significant correlation with PPF(r=0.252,P=0.024).The PPF in groups A and B(P=0.485),and groups C and D(P=0.184)did not show significant difference.However,the PPF in group F was significantly greater than that measured in group E(P=0.005).The PPFn of groups A,C,and E significantly decreased as the number of cycles increased(P=0.015).Meanwhile,the PPFn of groups B,D,and F remained consistent regardless of the number of cycles(P=0.367). The semidiameter of traditional pedicle screw insertion area was significantly larger than that of micro-dynamic pedicle screw insertion area(P<0.001).Conclusions This study compared the performance of a micro-dynamic pedicle screw versus that of a traditional pedicle screw using pull-out testing and micro-CT.The results showed that the micro-dynamic pedicle screw provides similar resistance in lowcycle fatigue testing and favorable resistance in high-cycle fatigue testing versus the traditional pedicle screw.The micro-dynamic pedicle screw provides stronger fixation stability versus the traditional pedicle screw,especially in high-cyclic fatigue loading condition versus the traditional pedicle screws.
基金This work was supported by the Fundamental Research Funds for the Central Universities(WK9110000143)the USTC Research Funds of the Double First-Class Initiative(YD9110002060)the Health Research Program of Anhui(AHWJ2022b060)。
文摘Objective:While age has been recognized as a noteworthy factor in preoperative graft selection,the correlation between age and hamstring autograft diameter has been the subject of continued debate within the scientific community.This study aimed to explore the correlation between the diameter of autologous tendon grafts and age in anterior cruciate ligament reconstruction(ACLR).Methods:A retrospective review of 388 patients who underwent arthroscopic ACLR with hamstring autografts was performed.Patients were grouped by age to analyze differences in hamstring autograft dia-meter and tendon cross-sectional area(CSA).We explored the correlations between age and graft diameter and between age and the CSA of the popliteal tendon while controlling for the influence of other pertinent variables.Results:Compared with female patients,male patients presented significantly greater autograft diameters and hamstring tendon CSAs(P<0.05).Notably,graft diameter and hamstring tendon CSA varied significantly across different age groups(P<0.05);patients aged>32 years were substantially more likely to have a graft diameter exceeding 8 mm and a CSA surpassing 18.5 mm^(2) than their≤32-year-old counterparts were(P<0.05).Conclusion:This study revealed that graft diameter varies across different age groups,with age independently influencing graft diameter.
基金supported by Basic and Applied Basic Research Fund Project of Guangdong(2022A1515011817,2023A1515030160)Research and Innovation Group of Guangdong University of Education(2024KYCXTD014)。
文摘The major problem with lithium-sulfur(Li-S)batteries is their poor cycling stability because of slow redox kinetics in the cathode and the growth of lithium dendrites on the anode.We report the production of 2D porous carbon nanosheets doped with both Fe and Ni(Fe/Ni-N-PCNSs)by an easy and template-free approach that solve this problem.Because of their ultrathin porous 2D structure and uniform distribution of Fe and Ni dopants,they capture polysulfides,speed up the sulfur redox reaction,and improve the material’s lithiophilicity,greatly suppressing the shuttling of polysulfides and dendrite growth on the lithium anode.As a result,it has an exceptional performance as a stable host for elemental sulfur and metallic lithium,producing a record long life of 1000 cycles with a very small capacity decay of 0.00025%per cycle in a Li-S battery and an excellent cycling stability of over 850 h with a small overpotential of>72 mV in a lithium metal battery.This work suggests the use of multifunctional-based 2D porous carbon nanosheets as a stable host for both elemental sulfur and metallic lithium to improve the Li-S battery per-formance.
